Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P11021:

ID   GRP78_HUMAN; STANDARD; 2DG.
AC   P11021;
DT   15-JAN-1999, integrated into SWISS-2DPAGE (release 9).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName:
DE   Full=Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; AltName:
DE   Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy
DE   chain-binding protein; Short=BiP; Flags: Precursor;.
GN   Name=HSPA5; Synonyms=GRP78;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, NUCLEI_LIVER_HUMAN.
IM   DLD1_HUMAN, NUCLEI_LIVER_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=20529951; PubMed=11079567; [NCBI, Expasy, EBI, Israel, Japan]
RA   Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'';
RL   Electrophoresis 21(1):3483-3487(2000).
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001DHC=4.98/75955;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001DHC: 986.574; 997.61; 1074.58; 1191.65;
2D         1228.68; 1329.6; 1460.77; 1566.79; 1887.95; 1934.01; 1999.04; 2164.92; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: NUCLEI_LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001IZA=4.97/71938;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001IZA: 997.486; 1191.615; 1313.6;
2D         1460.755; 1887.909; 1933.981; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P11021; P11021.
DR   DOSAC-COBS-2DPAGE; P11021; GRP78_HUMAN.
DR   OGP-WWW; P11021.
DR   PHCI-2DPAGE; P11021.
DR   PMMA-2DPAGE; P11021.
DR   REPRODUCTION-2DPAGE; P11021; P11021.
DR   Siena-2DPAGE; P11021; GRP78_HUMAN.
DR   UCD-2DPAGE; P11021; GRP78_HUMAN.
DR   UniProtKB/Swiss-Prot; P11021; GRP78_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal adenocarcinoma cell line (DL-1)	Soluble nuclear proteins and matrix from liver tissue

SWISS-2DPAGE (search AC)