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Sample Preparation and Post-separation Analysis

P11586:

ID   C1TC_HUMAN; STANDARD; 2DG.
AC   P11586;
DT   15-JAN-1999, integrated into SWISS-2DPAGE (release 9).
DT   01-OCT-2001, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 9.
DE   RecName: Full=C-1-tetrahydrofolate synthase, cytoplasmic; Short=C1-THF
DE   synthase; Includes: RecName: Full=Methylenetetrahydrofolate
DE   dehydrogenase; EC=1.5.1.5; Includes: RecName:
DE   Full=Methenyltetrahydrofolate cyclohydrolase; EC=3.5.4.9; Includes:
DE   RecName: Full=Formyltetrahydrofolate synthetase; EC=6.3.4.3;.
GN   Name=MTHFD1; Synonyms=MTHFC, MTHFD;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN.
IM   DLD1_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001D1X=6.79/125156;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001D1X: 878.455; 940.493; 983.569;
2D         1030.503; 1097.601; 1507.739; 2239.078; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
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DR   REPRODUCTION-2DPAGE; IPI00218342; IPI00218342.
DR   UniProtKB/Swiss-Prot; P11586; C1TC_HUMAN.
//

The following 2-D map is available for this protein:

	Colorectal adenocarcinoma cell line (DL-1)

SWISS-2DPAGE (search AC)