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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P13716



General information about the entry
View entry in simple text format
Entry nameHEM2_HUMAN
Primary accession numberP13716
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Delta-aminolevulinic acid dehydratase; Short=ALADH; EC=; AltName: Full=Porphobilinogen synthase;.
Gene nameName=ALAD
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P., Hochstrasser D.F.
''''''Plasma and red blood cell protein maps: update 1993'';'';''
Electrophoresis 14(1):1223-1231(1993)
2D PAGE maps for identified proteins
How to interpret a protein

RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
  map experimental info
  protein estimated location

pI=6.11; Mw=34997
pI=6.35; Mw=34783
pI=6.52; Mw=34711

MAPPING (identification):

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP13716; HEM2_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHEM2_HUMAN
Primary accession numberP13716
Secondary accession number(s) A8K375 B2R6F2 Q16870 Q16871 Q9BVQ9
Sequence was last modified on January 1, 1990 (version 1)
Annotations were last modified on October 19, 2011 (version 135)
Name and origin of the protein
DescriptionRecName: Full=Delta-aminolevulinic acid dehydratase; Short=ALADH; EC=; AltName: Full=Porphobilinogen synthase;
Gene nameName=ALAD
Encoded onName=ALAD
Keywords3D-structure; Allosteric enzyme; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Heme biosynthesis; Lyase; Metal-binding; Polymorphism; Porphyrin biosynthesis; Reference proteome; Zinc.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM13928; AAA51687.1; -; mRNA
EMBLX64467; CAA45796.1; -; Genomic_DNA
EMBLS99468; AAC60581.1; -; mRNA
EMBLS99471; AAC60582.1; -; mRNA
EMBLAK290490; BAF83179.1; -; mRNA
EMBLAK312552; BAG35449.1; -; mRNA
EMBLAY319481; AAP72012.1; -; Genomic_DNA
EMBLAL137066; CAH70099.3; -; Genomic_DNA
EMBLBC000977; AAH00977.3; ALT_INIT; mRNA
IPIIPI00442121; -; .
IPIIPI00790373; -; .
PIRA26478; A26478; .
RefSeqNP_000022.3; NM_000031.5; .
UniGeneHs.1227; -; .
PDB1E51; X-ray; 2.83 A; A/B=1-330
PDB1PV8; X-ray; 2.20 A; A/B=1-330
PDBsum1E51; -; .
PDBsum1PV8; -; .
ProteinModelPortalP13716; -; .
SMRP13716; 1-328; .
STRINGP13716; -; .
PhosphoSiteP13716; -; .
SWISS-2DPAGEP13716; -; .
OGPP13716; -; .
PRIDEP13716; -; .
EnsemblENST00000277315; ENSP00000277315; ENSG00000148218; .
EnsemblENST00000409155; ENSP00000386284; ENSG00000148218; .
GeneID210; -; .
KEGGhsa:210; -; .
NMPDRfig|9606.3.peg.31768; -; .
UCSCuc004bhm.2; human; .
CTD210; -; .
GeneCardsGC09M085755; -; .
H-InvDBHIX0008308; -; .
HPAHPA021023; -; .
HPAHPA022124; -; .
MIM125270; gene; .
MIM612740; phenotype; .
neXtProtNX_P13716; -; .
Orphanet100924; Porphyria due to ALA dehydratase deficiency; .
PharmGKBPA24687; -; .
eggNOGprNOG13729; -; .
GeneTreeENSGT00390000006998; -; .
HOVERGENHBG001222; -; .
OrthoDBEOG4BRWM1; -; .
PhylomeDBP13716; -; .
ReactomeREACT_9431; Metabolism of porphyrins; .
DrugBankDB00855; Aminolevulinic acid; .
NextBio840; -; .
ArrayExpressP13716; -; .
BgeeP13716; -; .
CleanExHS_ALAD; -; .
GenevestigatorP13716; -; .
GermOnlineENSG00000148218; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0042802; F:identical protein binding; IPI:UniProtKB; .
GOGO:0032791; F:lead ion binding; IDA:UniProtKB; .
GOGO:0004655; F:porphobilinogen synthase activity; IDA:UniProtKB; .
GOGO:0008270; F:zinc ion binding; IDA:UniProtKB; .
GOGO:0006783; P:heme biosynthetic process; IDA:UniProtKB; .
GOGO:0051260; P:protein homooligomerization; IPI:UniProtKB; .
InterProIPR001731; 4pyrrol_synth_porphobiln_synth; .
InterProIPR013785; Aldolase_TIM; .
Gene3DG3DSA:; Aldolase_TIM; 1; .
PANTHERPTHR11458; AlaD_dehydratase; 1; .
PfamPF00490; ALAD; 1; .
PIRSFPIRSF001415; Porphbilin_synth; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server