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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P17182




SWISS-2DPAGE:  P17182


P17182


General information about the entry
View entry in simple text format
Entry nameENOA_MOUSE
Primary accession numberP17182
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Alpha-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 1; AltName: Full=Non-neural enolase; Short=NNE;.
Gene nameName=Eno1
Synonyms=Eno-1
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=6.25; Mw=45708
pI=6.09; Mw=45565
pI=5.93; Mw=45565
pI=5.54; Mw=37928

MAPPING (identification):
SPOTS 2D-001P*: GEL MATCHING WITH WAT_MOUSE [2];
SPOT 2D-001UMQ: Peptide mass fingerprinting [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=6.16; Mw=47076
pI=6.34; Mw=46684  [identification data]

MAPPING (identification):
MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1];
SPOT 2D-0013IW: Peptide mass fingerprinting [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=6.07; Mw=47016
pI=6.24; Mw=46430

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
Lens-2DPAGEP17182.
REPRODUCTION-2DPAGEP17182; P17182.
REPRODUCTION-2DPAGEIPI00462072; IPI00462072.
UCD-2DPAGEP17182; ENOA_MOUSE.
UniProtKB/Swiss-ProtP17182; ENOA_MOUSE.
World-2DPAGE RepositoryP17182; ENOA_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameENOA_MOUSE
Primary accession numberP17182
Secondary accession number(s) Q99KT7 Q9DCY7
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 133)
Name and origin of the protein
DescriptionRecName: Full=Alpha-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 1; AltName: Full=Non-neural enolase; Short=NNE;
Gene nameName=Eno1
Synonyms=Eno-1
Encoded onName=Eno1; Synonyms=Eno-1
KeywordsAcetylation; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX52379; CAA36605.1; -; mRNA
EMBLAK002336; BAB22021.1; -; mRNA
EMBLBC003891; AAH03891.1; -; mRNA
EMBLBC004017; AAH04017.1; -; mRNA
EMBLBC010685; AAH10685.1; -; mRNA
EMBLBC024644; AAH24644.1; -; mRNA
EMBLBC085098; AAH85098.1; -; mRNA
IPIIPI00462072; -; .
PIRS10246; S10246; .
RefSeqNP_001020559.1; NM_001025388.1; .
RefSeqNP_075608.2; NM_023119.2; .
RefSeqXP_003086454.1; XM_003086406.1; .
RefSeqXP_003086455.1; XM_003086407.1; .
RefSeqXP_003086457.1; XM_003086409.1; .
RefSeqXP_003086458.1; XM_003086410.1; .
RefSeqXP_003086459.1; XM_003086411.1; .
RefSeqXP_003086596.1; XM_003086548.1; .
UniGeneMm.372357; -; .
UniGeneMm.372389; -; .
UniGeneMm.70666; -; .
ProteinModelPortalP17182; -; .
SMRP17182; 2-434; .
IntActP17182; 5; .
STRINGP17182; -; .
PhosphoSiteP17182; -; .
SWISS-2DPAGEP17182; -; .
REPRODUCTION-2DPAGEIPI00462072; -; .
REPRODUCTION-2DPAGEP17182; -; .
UCD-2DPAGEP17182; -; .
PRIDEP17182; -; .
EnsemblENSMUST00000076155; ENSMUSP00000075513; ENSMUSG00000059040; .
EnsemblENSMUST00000080926; ENSMUSP00000079727; ENSMUSG00000063524; .
GeneID100045967; -; .
GeneID100503183; -; .
GeneID13806; -; .
GeneID433182; -; .
KEGGmmu:100045967; -; .
KEGGmmu:100503183; -; .
KEGGmmu:13806; -; .
KEGGmmu:433182; -; .
CTD2023; -; .
MGIMGI:95393; Eno1; .
eggNOGroNOG10078; -; .
HOGENOMHBG726599; -; .
HOVERGENHBG000067; -; .
InParanoidP17182; -; .
OMAKVEIGMD; -; .
OrthoDBEOG4T783B; -; .
PhylomeDBP17182; -; .
BRENDA4.2.1.11; 3474; .
NextBio457726; -; .
BgeeP17182; -; .
GenevestigatorP17182; -; .
GermOnlineENSMUSG00000059040; Mus musculus; .
GermOnlineENSMUSG00000063524; Mus musculus; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IEA:EC; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server