resource logo

SWISS-2DPAGE

Attention: World-2DPAGE is no longer maintained.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P18335




SWISS-2DPAGE:  P18335


P18335


General information about the entry
View entry in simple text format
Entry nameARGD_ECOLI
Primary accession numberP18335
integrated into SWISS-2DPAGE on April 1, 2000 (release 12)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=Acetylornithine/succinyldiaminopimelate aminotransferase; Short=ACOAT; Short=DapATase; Short=Succinyldiaminopimelate transferase; EC=2.6.1.11; EC=2.6.1.17;.
Gene nameName=argD
Synonyms=dapC, dtu
OrderedLocusNames=b3359, JW3322
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.19; Mw=33559  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.75; Mw=41241  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP18335; ARGD_ECOLI.
UniProtKB/Swiss-ProtP18335; ARGD_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameARGD_ECOLI
Primary accession numberP18335
Secondary accession number(s) Q2M725
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on October 19, 2011 (version 109)
Name and origin of the protein
DescriptionRecName: Full=Acetylornithine/succinyldiaminopimelate aminotransferase; Short=ACOAT; Short=DapATase; Short=Succinyldiaminopimelate transferase; EC=2.6.1.11; EC=2.6.1.17;
Gene nameName=argD
Synonyms=dapC, dtu
OrderedLocusNames=b3359, JW3322
Encoded onName=argD; Synonyms=dapC, dtu; OrderedLocusNames=b3359, JW3322
KeywordsAmino-acid biosynthesis; Aminotransferase; Arginine biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; Lysine biosynthesis; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM32796; AAA23480.1; -; Genomic_DNA
EMBLU18997; AAA58156.1; -; Genomic_DNA
EMBLU00096; AAC76384.1; -; Genomic_DNA
EMBLAP009048; BAE77931.1; -; Genomic_DNA
PIRB65130; B65130; .
RefSeqNP_417818.1; NC_000913.2; .
ProteinModelPortalP18335; -; .
SMRP18335; 18-404; .
DIPDIP-9138N; -; .
IntActP18335; 6; .
SWISS-2DPAGEP18335; -; .
2DBase-EcoliP18335; -; .
EnsemblBacteriaEBESCT00000000470; EBESCP00000000470; EBESCG00000000390; .
EnsemblBacteriaEBESCT00000016195; EBESCP00000015486; EBESCG00000015255; .
GeneID947864; -; .
GenomeReviewsAP009048_GR; JW3322; .
GenomeReviewsU00096_GR; b3359; .
KEGGecj:JW3322; -; .
KEGGeco:b3359; -; .
EchoBASEEB0064; -; .
EcoGeneEG10066; argD; .
eggNOGCOG4992; -; .
GeneTreeEBGT00050000010246; -; .
HOGENOMHBG725944; -; .
OMAERTRVIT; -; .
ProtClustDBPRK05093; -; .
BioCycEcoCyc:ACETYLORNTRANSAM-MONOMER; -; .
BioCycMetaCyc:ACETYLORNTRANSAM-MONOMER; -; .
GenevestigatorP18335; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0003992; F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity; IDA:EcoliWiki; .
GOGO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GOGO:0009016; F:succinyldiaminopimelate transaminase activity; IDA:EcoliWiki; .
GOGO:0006526; P:arginine biosynthetic process; IDA:EcoCyc; .
GOGO:0009085; P:lysine biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_01107; ArgD_aminotrans_3; 1; -
InterProIPR017652; Ac/SuccinylOrn_transaminase; .
InterProIPR004636; AcOrn/SuccinylOrn_aminoTrfase; .
InterProIPR005814; Aminotrans_3; .
InterProIPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterProIPR015421; PyrdxlP-dep_Trfase_major_sub1; .
InterProIPR015422; PyrdxlP-dep_Trfase_major_sub2; .
Gene3DG3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
Gene3DG3DSA:3.90.1150.10; PyrdxlP-dep_Trfase_major_sub2; 2; .
PANTHERPTHR11986; Aminotrans_3; 1; .
PANTHERPTHR11986:SF19; ArgD_aminotrans; 1; .
PfamPF00202; Aminotran_3; 1; .
SUPFAMSSF53383; PyrdxlP-dep_Trfase_major; 1; .
TIGRFAMsTIGR03246; Arg_catab_astC; 1; .
TIGRFAMsTIGR00707; ArgD; 1; .
PROSITEPS00600; AA_TRANSFER_CLASS_3; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server