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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P20029




SWISS-2DPAGE:  P20029


P20029


General information about the entry
View entry in simple text format
Entry nameGRP78_MOUSE
Primary accession numberP20029
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; Flags: Precursor;.
Gene nameName=Hspa5
Synonyms=Grp78
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, Expasy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=4.96; Mw=65750
pI=5.00; Mw=65625
pI=5.03; Mw=65500
pI=5.06; Mw=65500
pI=5.09; Mw=65376

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [2].



ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=5.03; Mw=74415  [identification data]
pI=5.06; Mw=74028  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=4.99; Mw=70356  [identification data]
pI=5.01; Mw=70169
pI=5.04; Mw=69426  [identification data]

MAPPING (identification):
MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1]; SPOTS 2D-00132H, 2D-00132M: Peptide mass fingerprinting [2].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=4.98; Mw=72540
pI=5.01; Mw=72138
pI=5.04; Mw=71938  [identification data]

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2];
SPOT 2D-0016JI: Peptide mass fingerprinting [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=5.06; Mw=68965
pI=5.00; Mw=69796
pI=5.03; Mw=69241
pI=5.08; Mw=69518

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP20029; GRP78_MOUSE.
REPRODUCTION-2DPAGEP20029; P20029.
REPRODUCTION-2DPAGEIPI00319992; IPI00319992.
REPRODUCTION-2DPAGEIPI00351252; IPI00351252.
REPRODUCTION-2DPAGEIPI00880839; IPI00880839.
UCD-2DPAGEP20029; GRP78_MOUSE.
UniProtKB/Swiss-ProtP20029; GRP78_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGRP78_MOUSE
Primary accession numberP20029
Secondary accession number(s) O35642 Q3UFF2 Q61630
Sequence was last modified on November 1, 1997 (version 3)
Annotations were last modified on October 19, 2011 (version 124)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein; Short=GRP-78; AltName: Full=Heat shock 70 kDa protein 5; AltName: Full=Immunoglobulin heavy chain-binding protein; Short=BiP; Flags: Precursor;
Gene nameName=Hspa5
Synonyms=Grp78
Encoded onName=Hspa5; Synonyms=Grp78
KeywordsAcetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAJ002387; CAA05361.1; -; mRNA
EMBLM19351; AAA37315.1; -; mRNA
EMBLD78645; BAA11462.1; -; mRNA
EMBLAK076079; BAC36166.1; -; mRNA
EMBLAK146647; BAE27328.1; -; mRNA
EMBLAK148539; BAE28609.1; -; mRNA
EMBLAK151647; BAE30576.1; -; mRNA
EMBLAK152020; BAE30882.1; -; mRNA
EMBLAK166739; BAE38982.1; -; mRNA
EMBLAK169034; BAE40825.1; -; mRNA
EMBLBC050927; AAH50927.1; -; mRNA
EMBLU16277; AAA76734.1; -; Genomic_DNA
EMBLM30779; AAA37742.1; -; mRNA
IPIIPI00319992; -; .
PIRA37048; A37048; .
RefSeqNP_001156906.1; NM_001163434.1; .
RefSeqNP_071705.3; NM_022310.3; .
UniGeneMm.330160; -; .
UniGeneMm.470180; -; .
ProteinModelPortalP20029; -; .
SMRP20029; 27-641; .
DIPDIP-32341N; -; .
IntActP20029; 6; .
MINTMINT-1177274; -; .
STRINGP20029; -; .
PhosphoSiteP20029; -; .
SWISS-2DPAGEP20029; -; .
COMPLUYEAST-2DPAGEP20029; -; .
REPRODUCTION-2DPAGEIPI00319992; -; .
REPRODUCTION-2DPAGEP20029; -; .
UCD-2DPAGEP20029; -; .
PRIDEP20029; -; .
EnsemblENSMUST00000028222; ENSMUSP00000028222; ENSMUSG00000026864; .
EnsemblENSMUST00000100171; ENSMUSP00000097747; ENSMUSG00000026864; .
GeneID14828; -; .
KEGGmmu:14828; -; .
CTD3309; -; .
MGIMGI:95835; Hspa5; .
HOGENOMHBG334976; -; .
HOVERGENHBG051845; -; .
InParanoidP20029; -; .
OMANQLTTNP; -; .
OrthoDBEOG444KJV; -; .
PhylomeDBP20029; -; .
ReactomeREACT_89750; Hemostasis; .
ArrayExpressP20029; -; .
BgeeP20029; -; .
CleanExMM_HSPA5; -; .
GenevestigatorP20029; -; .
GermOnlineENSMUSG00000026864; Mus musculus; .
GOGO:0009986; C:cell surface; IDA:MGI; .
GOGO:0005788; C:endoplasmic reticulum lumen; IDA:BHF-UCL; .
GOGO:0005576; C:extracellular region; TAS:Reactome; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051787; F:misfolded protein binding; IDA:BHF-UCL; .
GOGO:0043022; F:ribosome binding; IDA:MGI; .
GOGO:0006987; P:activation of signaling protein activity involved in unfolded protein response; IMP:BHF-UCL; .
GOGO:0021680; P:cerebellar Purkinje cell layer development; IMP:BHF-UCL; .
GOGO:0021589; P:cerebellum structural organization; IMP:BHF-UCL; .
GOGO:0006983; P:ER overload response; IDA:MGI; .
GOGO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IGI:MGI; .
GOGO:0040019; P:positive regulation of embryonic development; TAS:BHF-UCL; .
GOGO:0031398; P:positive regulation of protein ubiquitination; IMP:BHF-UCL; .
GOGO:0051603; P:proteolysis involved in cellular protein catabolic process; IDA:BHF-UCL; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server