Sample Preparation and Post-separation Analysis

P20152:

ID   VIME_MOUSE; STANDARD; 2DG.
AC   P20152;
DT   01-SEP-1998, integrated into SWISS-2DPAGE (release 7).
DT   01-OCT-2001, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 8.
DE   RecName: Full=Vimentin;.
GN   Name=Vim;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
MT   BAT_MOUSE, ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, NUCLEI_LIVER_MOUSE, WAT_MOUSE.
IM   BAT_MOUSE, ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, NUCLEI_LIVER_MOUSE, WAT_MOUSE.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96154592; PubMed=8586073; [NCBI, Expasy, EBI, Israel, Japan]
RA   Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers
RA   J., Steiner S., Witzmann F., Anderson N.G.;
RT   ''''''An updated two-dimensional gel database of rat liver proteins
RT   useful in gene regulation and drug effect studies'';'';'';
RL   Electrophoresis 16(1):1977-1981(1995).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano
RA   S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A.,
RA   Hochstrasser D.F.;
RT   ''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of
RT   diabetes and obesity'';'';'';
RL   Proteomics 1(1):136-163(2001).
2D   -!- MASTER: BAT_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001Q0T=4.85/38681; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001Q0T: 906.558; 1062.631; 1093.659;
2D         1115.673; 1169.772; 1270.699; 1311.736; 1320.733; 1533.921; 1839.042;
2D         2217.073; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ISLETS_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0018H2=5.11/59488;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0018H2: 1296.619; 1444.731; 1495.831;
2D         1557.92; 2216.975; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: LIVER_MOUSE;
2D   -!-   PI/MW: SPOT 2D-00138C=5.16/59607;
2D   -!-   PI/MW: SPOT 2D-0013E4=5.13/53059;
2D   -!-   PEPTIDE MASSES: SPOT 2D-00138C: 905.471; 925.473; 1093.542;
2D         1270.62; 1296.637; 1309.646; 1320.651; 1433.739; 1444.696; 1495.758;
2D         1533.809; 1557.902; 1838.892; 2200.937; 2216.903; TRYPSIN.
2D   -!-   MAPPING: SPOT 2D-00138C: Peptide mass fingerprinting [2]; SPOT
2D         2D-0013E4: MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1].
2D   -!- MASTER: MUSCLE_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0016SR=5.14/55496;
2D   -!-   MAPPING: MATCHING WITH THE MOUSE LIVER MASTER GEL [2].
2D   -!- MASTER: NUCLEI_LIVER_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001NCG=5.09/55195;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001NCG: 1320.614; 1444.727; 1495.777;
2D         1557.934; 1766.82; 1838.944; 2423.131; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: WAT_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001B0W=5.12/53967;
2D   -!-   PI/MW: SPOT 2D-001BI9=4.92/39581; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001BI9: 870.422; 906.463; 1060.553;
2D         1062.543; 1093.512; 1115.56; 1118.5; 1121.567; 1169.668; 1270.561;
2D         1296.619; 1320.591; 1533.829; TRYPSIN.
2D   -!-   MAPPING: SPOT 2D-001B0W: MATCHING WITH THE MOUSE LIVER MASTER GEL
2D         [2]; SPOT 2D-001BI9: Peptide mass fingerprinting [2].
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DR   REPRODUCTION-2DPAGE; IPI00227299; IPI00227299.
DR   UCD-2DPAGE; P20152; VIME_MOUSE.
DR   UniProtKB/Swiss-Prot; P20152; VIME_MOUSE.
DR   World-2DPAGE Repository; P20152; VIME_MOUSE.
//

The following 2-D maps are available for this protein:

	Brown adipose tissue	Pancreatic islet cells	Liver
	Gastrocnemius muscle	Soluble nuclear proteins and matrix from liver tissue	White adipose tissue

SWISS-2DPAGE (search AC)