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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P20674




SWISS-2DPAGE: P20674



P20674:

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ID   COX5A_HUMAN; STANDARD; 2DG.
AC   P20674;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName:
DE   Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor;.
GN   Name=COX5A;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
MT   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, PLATELET_HUMAN.
IM   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
IM   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, PLATELET_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot
RA   J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.;
RT   ''''''Human liver protein map: update 1993'';'';'';
RL   Electrophoresis 14(1):1216-1222(1993).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant
RA   L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.;
RT   ''''''Human blood platelet protein map established by two-dimensional
RT   polyacrylamide gel electrophoresis'';'';'';
RL   Electrophoresis 16(1):1152-1159(1995).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [5]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [6]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [7]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
RN   [8]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [9]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC   -!- SUBUNIT: ONE OF THE NUCLEAR-CODED POLYPEPTIDE CHAINS OF CYTOCHROME C
CC         OXIDASE
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000UZ5=4.80/12121;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [7].
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001F8S=4.93/11055;
2D   -!-   MAPPING: MATCHING WITH THE HEPG2 MASTER GEL [8].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000ETL=4.82/9514;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0003YO=4.93/11646;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00104G=4.90/11355;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [5][6].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000O0T=4.90/12400;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000O0T: B=12.7, Z=14.1, S=3.4,
2D         H=1.1, G=6.5, T=6, A=7.2, P=7.1, Y=3.9, R=6.9, V=7.2, M=0.5, I=6.3,
2D         L=9.2, F=3.2, K=4.9;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID
2D         COMPOSITION ANALYSIS [4].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001OG=4.90/12293;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YG3=4.75/16427;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001YG3: 1607.6572; 1692.8538; 2052.9844;
2D         3428.8217; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [9].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0008CD=4.89/12353;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: PLATELET_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000GJ5=4.91/12343;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P20674; P20674.
DR   DOSAC-COBS-2DPAGE; P20674; COX5A_HUMAN.
DR   HSC-2DPAGE; P20674; HUMAN.
DR   OGP-WWW; P20674.
DR   Siena-2DPAGE; P20674; COX5A_HUMAN.
DR   UCD-2DPAGE; P20674; COX5A_HUMAN.
DR   UniProtKB/Swiss-Prot; P20674; COX5A_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal epithelia cells
CEC_HUMAN
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2
HEPG2_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Liver
LIVER_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Lymphoma
LYMPHOMA_HUMAN
Platelet
PLATELET_HUMAN
  


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server