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Sample Preparation and Post-separation Analysis


Searching in 'SWISS-2DPAGE' for entry matching: P23254




SWISS-2DPAGE:  P23254

P23254


General information about the entry
View entry in simple text format
Entry nameTKT1_YEAST
Primary accession numberP23254
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Transketolase 1; Short=TK 1; EC=2.2.1.1;.
Gene nameName=TKL1
OrderedLocusNames=YPR074C
ORFNames=YP9499.29C
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=95203288; PubMed=7895733; [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';''
Electrophoresis 15(1):1466-1486(1994)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location 		 
YEAST

MAP LOCATIONS:
pI=6.64; Mw=73000
pI=6.70; Mw=73000
pI=6.76; Mw=72795

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED BY GARRELS [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP23254; TKT1_YEAST.
YEPD4776.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTKT1_YEAST
Primary accession numberP23254
Secondary accession number(s) D6W478
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on October 19, 2011 (version 133)
Name and origin of the protein
DescriptionRecName: Full=Transketolase 1; Short=TK 1; EC=2.2.1.1;
Gene nameName=TKL1
OrderedLocusNames=YPR074C
ORFNames=YP9499.29C
Encoded onName=TKL1; OrderedLocusNames=YPR074C; ORFNames=YP9499.29C
Keywords3D-structure; Calcium; Complete proteome; Direct protein sequencing; Metal-binding; Phosphoprotein; Reference proteome; Thiamine pyrophosphate; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM63302; AAA35168.1; -; Genomic_DNA
EMBLX73224; CAA51693.1; -; Genomic_DNA
EMBLZ49219; CAA89191.1; -; Genomic_DNA
EMBLZ71255; CAA94982.1; -; Genomic_DNA
EMBLU51033; AAB68125.1; -; Genomic_DNA
EMBLBK006949; DAA11494.1; -; Genomic_DNA
PIRA49510; XJBYTK; .
RefSeqNP_015399.1; NM_001184171.1; .
PDB1AY0; X-ray; 2.60 A; A/B=1-680
PDB1GPU; X-ray; 1.86 A; A/B=2-679
PDB1NGS; X-ray; 2.40 A; A/B=2-679
PDB1TKA; X-ray; 2.70 A; A/B=3-679
PDB1TKB; X-ray; 2.30 A; A/B=3-679
PDB1TKC; X-ray; 2.70 A; A/B=3-679
PDB1TRK; X-ray; 2.00 A; A/B=1-680
PDBsum1AY0; -; .
PDBsum1GPU; -; .
PDBsum1NGS; -; .
PDBsum1TKA; -; .
PDBsum1TKB; -; .
PDBsum1TKC; -; .
PDBsum1TRK; -; .
ProteinModelPortalP23254; -; .
SMRP23254; 3-680; .
DIPDIP-6765N; -; .
IntActP23254; 8; .
MINTMINT-629387; -; .
STRINGP23254; -; .
SWISS-2DPAGEP23254; -; .
PeptideAtlasP23254; -; .
EnsemblFungiYPR074C; YPR074C; YPR074C; .
GeneID856188; -; .
KEGGsce:YPR074C; -; .
NMPDRfig|4932.3.peg.6535; -; .
SGDS000006278; TKL1; .
eggNOGfuNOG07475; -; .
GeneTreeEFGT00050000002270; -; .
HOGENOMHBG565885; -; .
OMARDGFPVV; -; .
OrthoDBEOG4V46GV; -; .
PhylomeDBP23254; -; .
NextBio981372; -; .
ArrayExpressP23254; -; .
GenevestigatorP23254; -; .
GermOnlineYPR074C; Saccharomyces cerevisiae; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0004802; F:transketolase activity; IMP:SGD; .
GOGO:0006098; P:pentose-phosphate shunt; IMP:SGD; .
InterProIPR009014; Transketo_C/Pyr-ferredox_oxred; .
InterProIPR015941; Transketolase-like_C; .
InterProIPR005475; Transketolase-like_Pyr-bd; .
InterProIPR005478; Transketolase_bac-like; .
InterProIPR020826; Transketolase_BS; .
InterProIPR005476; Transketolase_C; .
InterProIPR005474; Transketolase_N; .
Gene3DG3DSA:3.40.50.920; Transketo_C_like; 1; .
PfamPF02779; Transket_pyr; 1; .
PfamPF02780; Transketolase_C; 1; .
PfamPF00456; Transketolase_N; 1; .
SMARTSM00861; Transket_pyr; 1; .
SUPFAMSSF52922; Transketo_C_like; 1; .
TIGRFAMsTIGR00232; Tktlase_bact; 1; .
PROSITEPS00801; TRANSKETOLASE_1; 1; .
PROSITEPS00802; TRANSKETOLASE_2; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server