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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P25553 |
SWISS-2DPAGE: P25553
P25553:
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ID ALDA_ECOLI; STANDARD; 2DG.
AC P25553;
DT 01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 13.
DE RecName: Full=Lactaldehyde dehydrogenase; EC=1.2.1.22; AltName:
DE Full=Aldehyde dehydrogenase A; AltName: Full=Glycolaldehyde
DE dehydrogenase; EC=1.2.1.21;.
GN Name=aldA; Synonyms=ald; OrderedLocusNames=b1415, JW1412;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-001508=5.29/42560;
2D -!- PI/MW: SPOT 2D-00153A=5.53/38941;
2D -!- PEPTIDE MASSES: SPOT 2D-001508: 974.812; 999.727; 1057.836;
2D 1111.813; 1570.196; 1669.201; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-00153A: 974.771; 999.476; 1011.57;
2D 1111.538; 1299.61; 1506.673; 1569.773; 1684.803; 1733.777; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001L6J=5.43/50034;
2D -!- PEPTIDE MASSES: SPOT 2D-001L6J: 974.59; 999.502; 1011.554;
2D 1111.541; 1569.825; 2233.091; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WIC=5.11/52363;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WIC: (R)AQPEWEALPAIER(A),55-67;
2D (R)ASEISALIVEEGGK(I),82-95; (R)RYEGEIIQSDRPGENILLFK(R),120-139;
2D (R)GVFNLVLGR(G),199-207; (R)GETVGQELAGNPK(V),208-220;
2D (K)GIYDQFVNR(L),295-303; (K)GYYYPPTLLLDVR(Q),361-373; (K)FGETYINR(E),431-438.
2D -!- MAPPING: Tandem mass spectrometry [2].
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DR UniProtKB/Swiss-Prot; P25553; ALDA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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