Sample Preparation and Post-separation Analysis

P30084:

ID   ECHM_HUMAN; STANDARD; 2DG.
AC   P30084;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   01-DEC-2000, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Enoyl-CoA hydratase, mitochondrial; EC=4.2.1.17; AltName:
DE   Full=Enoyl-CoA hydratase 1; AltName: Full=Short-chain enoyl-CoA
DE   hydratase; Short=SCEH; Flags: Precursor;.
GN   Name=ECHS1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, ELC_HUMAN, LIVER_HUMAN, LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
IM   CEC_HUMAN, ELC_HUMAN, LIVER_HUMAN, LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
CC   -!- SUBUNIT: HOMOHEXAMER 
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000UJX=5.95/25821;
2D   -!-   MAPPING: MICROSEQUENCING [4].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000EP9=6.21/24544;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001BO=5.92/26179;
2D   -!-   PI/MW: SPOT 2D-0001BW=5.57/26120;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00083E=5.91/26093;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0006I0=5.93/26000;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D40=5.86/26133;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
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DR   DOSAC-COBS 2D-PAGE; P30084; P30084.
DR   DOSAC-COBS-2DPAGE; P30084; ECHM_HUMAN.
DR   REPRODUCTION-2DPAGE; P30084; P30084.
DR   REPRODUCTION-2DPAGE; IPI00024993; IPI00024993.
DR   Siena-2DPAGE; P30084; ECHM_HUMAN.
DR   UCD-2DPAGE; P30084; ECHM_HUMAN.
DR   UniProtKB/Swiss-Prot; P30084; ECHM_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal epithelia cells	Erythroleukemia Cell	Liver
	Lymphoma	Red blood cells	Macrophage Like Cell Line

SWISS-2DPAGE (search AC)