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Sample Preparation and Post-separation Analysis

P30085:

ID   KCY_HUMAN; PRELIMINARY; 2DG.
AC   P30085;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 16.
DE   RecName: Full=UMP-CMP kinase; EC=2.7.4.14; AltName: Full=Cytidine
DE   monophosphate kinase; AltName: Full=Cytidylate kinase; AltName:
DE   Full=Deoxycytidylate kinase; AltName: Full=Uridine monophosphate kinase;
DE   AltName: Full=Uridine monophosphate/cytidine monophosphate kinase;
DE   Short=UMP/CMP kinase; Short=UMP/CMPK;.
GN   Name=CMPK1; Synonyms=CMK, CMPK, UCK, UMK, UMPK;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, ELC_HUMAN, HEPG2_HUMAN, LIVER_HUMAN, LYMPHOMA_HUMAN, U937_HUMAN.
IM   CEC_HUMAN, ELC_HUMAN, HEPG2_HUMAN, LIVER_HUMAN, LYMPHOMA_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000UQW=5.42/21422;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL AND MICROSEQUENCING [3].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000EQR=5.42/21616;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0003RI=5.41/21859;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001FV=5.37/21816;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00086T=5.43/21387;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D59=5.41/21450;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
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DR   OGP-WWW; P30085.
DR   Siena-2DPAGE; P30085; KCY_HUMAN.
DR   UniProtKB/Swiss-Prot; P30085; KCY_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal epithelia cells	Erythroleukemia Cell	HepG2
	Liver	Lymphoma	Macrophage Like Cell Line

SWISS-2DPAGE (search AC)