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Sample Preparation and Post-separation Analysis

P30859:

ID   ARTI_ECOLI; STANDARD; 2DG.
AC   P30859;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Putative ABC transporter arginine-binding protein 2; Flags: Precursor;.
GN   Name=artI; OrderedLocusNames=b0863, JW0847;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LFK=5.23/26630;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (AETI) [1].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LHF=5.07/31478;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LHF: 1045.656; 1203.684; 1268.805;
2D         1675.07; 1712.13; 1863.1; 2300.463; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WTV=5.27/24964;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WTV: 953.43; 1138.56; 1168.58; 1711.93;
2D         1792.85; 1814.93; 2376.2; 2633.3; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WTV:
2D         (K)QVLFTTPYYDNSALFVGQQGK(Y),97-117; (K)YTSVDQLK(G),118-125;
2D         (K)VTDKDYFGTGLGIAVR(Q),195-210; (R)QGNTELQQKLNTALEK(V),211-226.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [3].
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [3].
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DR   UniProtKB/Swiss-Prot; P30859; ARTI_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli(5-6)	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)