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Sample Preparation and Post-separation Analysis

P32080:

ID   URA6_HUMAN; PRELIMINARY; 2DG.
AC   P32080;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   01-DEC-2000, 2D annotation version 1.
DT   26-SEP-2006, general annotation version 8.
DE   Unknown protein from 2D-PAGE of red blood cells (Spot 17) (Fragment).
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   PLATELET_HUMAN, RBC_HUMAN.
IM   PLATELET_HUMAN, RBC_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007935; PubMed=7498158; [NCBI, Expasy, EBI, Israel, Japan]
RA   Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant
RA   L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.;
RT   ''''''Human blood platelet protein map established by two-dimensional
RT   polyacrylamide gel electrophoresis'';'';'';
RL   Electrophoresis 16(1):1152-1159(1995).
2D   -!- MASTER: PLATELET_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000FJV=5.36/65079;
2D   -!-   PI/MW: SPOT 2D-000FK6=5.32/65079;
2D   -!-   MAPPING: MATCHING WITH THE RBC MASTER GEL [2].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0005VG=5.32/72238;
2D   -!-   PI/MW: SPOT 2D-0005VH=5.38/71951;
2D   -!-   MAPPING: MICROSEQUENCING (ASEAHXGAVN) [1].
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DR   UniProtKB/Swiss-Prot; P32080; URA6_HUMAN.
//

The following 2-D maps are available for this protein:

	Platelet	Red blood cells

SWISS-2DPAGE (search AC)