Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P32119:

ID   PRDX2_HUMAN; STANDARD; 2DG.
AC   P32119; P32118;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 10.
DE   RecName: Full=Peroxiredoxin-2; EC=1.11.1.15; AltName: Full=Natural killer
DE   cell-enhancing factor B; Short=NKEF-B; AltName: Full=PRP; AltName:
DE   Full=Thiol-specific antioxidant protein; Short=TSA; AltName:
DE   Full=Thioredoxin peroxidase 1; AltName: Full=Thioredoxin-dependent
DE   peroxide reductase 1;.
GN   Name=PRDX2; Synonyms=NKEFB, TDPX1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, LYMPHOCYTE_HUMAN, NUCLEOLI_HELA_2D_HUMAN, RBC_HUMAN.
IM   DLD1_HUMAN, LYMPHOCYTE_HUMAN, NUCLEOLI_HELA_2D_HUMAN, RBC_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [2]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
RN   [4]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001EWB=5.38/21646;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001EWB: 972.658; 1179.765; 1211.806;
2D         1334.906; 1929.196; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YEJ=5.41/22317;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001YEJ: 862.50838; 924.46007; 972.54725;
2D         978.50386; 1023.5095; 1179.6358; 1211.6889; 1734.985; 1863.0792; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VZP=5.70/24026;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VZP: 924.5; 972.6; 1023.5; 1179.7;
2D         1211.7; 1287.8; 1735.08; 1863.2; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001VZP: (R)QITVNDLPVGR(S),140-150.
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [3].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0006LC=5.41/21321;
2D   -!-   MAPPING: INTERNAL SEQUENCE (TDEGIATTGL) [1].
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DR   DOSAC-COBS 2D-PAGE; P32119; P32119.
DR   DOSAC-COBS-2DPAGE; P32119; PRDX2_HUMAN.
DR   OGP-WWW; P32119.
DR   REPRODUCTION-2DPAGE; IPI00027350; IPI00027350.
DR   UCD-2DPAGE; P32119; PRDX2_HUMAN.
DR   UniProtKB/Swiss-Prot; P32119; PRDX2_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal adenocarcinoma cell line (DL-1)	Lymphocytes	2D-PAGE of nucleolar proteins from Human HeLa cells
	Red blood cells

SWISS-2DPAGE (search AC)