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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P38489 SWISS-2DPAGE:  P38489 P38489 General information about the entry View entry in simple text format Entry name NFNB_ECOLI Primary accession number P38489 integrated into SWISS-2DPAGE on  October 1, 1998 (release 8) 2D Annotations were last modified on March 31, 2004 (version 4) General Annotations were last modified on  May 19, 2011 (version 12) Name and origin of the protein Description RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase;. Gene name Name=nfnB Synonyms=dprA, nfsB, nfsI, ntr OrderedLocusNames=b0578, JW0567 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan] Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C. ''''''New perspectives in the Escherichia coli proteome investigation'';'';'' Proteomics 1(1):409-423(2001) [2]   MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) 2D PAGE maps for identified proteins How to interpret a protein ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI4.5-5.5 MAP LOCATIONS: SPOT 2D-001HEN: pI=5.55; Mw=25092   [identification data] SPOT 2D-0015EU: pI=5.09; Mw=14160   [identification data] MAPPING (identification): Peptide mass fingerprinting [1]. ECOLI5-6 {Escherichia coli(5-6)} Escherichia coli   map experimental info   protein estimated location   ECOLI5-6 MAP LOCATIONS: SPOT 2D-001LGL: pI=5.47; Mw=32502   [identification data] SPOT 2D-001LKH: pI=5.32; Mw=28018   [identification data] MAPPING (identification): Peptide mass fingerprinting [1]. ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WU3: pI=5.83; Mw=24964   [identification data] MAPPING (identification): Peptide mass fingerprinting and tandem mass spectrometry [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references UniProtKB/Swiss-Prot P38489; NFNB_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name NFNB_ECOLI Primary accession number P38489 Secondary accession number(s) P19575 Sequence was last modified on  October 1, 1994 (version 1) Annotations were last modified on  October 19, 2011 (version 101) Name and origin of the protein Description RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase; Gene name Name=nfnB Synonyms=dprA, nfsB, nfsI, ntr OrderedLocusNames=b0578, JW0567 Encoded on Name=nfnB; Synonyms=dprA, nfsB, nfsI, ntr; OrderedLocusNames=b0578, JW0567 Keywords 3D-structure; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; NAD; NADP; Oxidoreductase; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL D25414; BAA05004.1; -; Genomic_DNA EMBL U07860; AAC43263.1; -; Genomic_DNA EMBL U82598; AAB40776.1; -; Genomic_DNA EMBL U00096; AAC73679.1; -; Genomic_DNA EMBL AP009048; BAA35218.1; -; Genomic_DNA PIR I67685; I67685; . PIR S01818; S01818; . RefSeq NP_415110.1; NC_000913.2; . PDB 1DS7; X-ray; 2.06 A; A/B=1-217 PDB 1ICR; X-ray; 1.70 A; A/B=1-217 PDB 1ICU; X-ray; 1.80 A; A/B/C/D=1-217 PDB 1ICV; X-ray; 2.40 A; A/B/C/D=1-217 PDB 1IDT; X-ray; 2.00 A; A/B=1-217 PDB 1OO5; X-ray; 2.50 A; A/B=1-217 PDB 1OO6; X-ray; 2.00 A; A/B=1-217 PDB 1OON; X-ray; 2.49 A; A/B=1-217 PDB 1OOQ; X-ray; 2.00 A; A/B=1-217 PDB 1YKI; X-ray; 1.70 A; A/B/C/D=1-217 PDB 1YLR; X-ray; 1.70 A; A/B=1-217 PDB 1YLU; X-ray; 2.00 A; A/B=1-217 PDBsum 1DS7; -; . PDBsum 1ICR; -; . PDBsum 1ICU; -; . PDBsum 1ICV; -; . PDBsum 1IDT; -; . PDBsum 1OO5; -; . PDBsum 1OO6; -; . PDBsum 1OON; -; . PDBsum 1OOQ; -; . PDBsum 1YKI; -; . PDBsum 1YLR; -; . PDBsum 1YLU; -; . ProteinModelPortal P38489; -; . SMR P38489; 1-217; . DIP DIP-10330N; -; . IntAct P38489; 12; . SWISS-2DPAGE P38489; -; . EnsemblBacteria EBESCT00000003087; EBESCP00000003087; EBESCG00000002529; . EnsemblBacteria EBESCT00000014539; EBESCP00000013830; EBESCG00000013600; . GeneID 945778; -; . GenomeReviews AP009048_GR; JW0567; . GenomeReviews U00096_GR; b0578; . KEGG ecj:JW0567; -; . KEGG eco:b0578; -; . EchoBASE EB4146; -; . EcoGene EG20151; nfnB; . eggNOG COG0778; -; . GeneTree EBGT00050000010591; -; . HOGENOM HBG751302; -; . OMA KRYSTKA; -; . ProtClustDB PRK11053; -; . BioCyc EcoCyc:DIHYDROPTERIREDUCT-MONOMER; -; . BioCyc MetaCyc:DIHYDROPTERIREDUCT-MONOMER; -; . DrugBank DB01093; Dimethyl sulfoxide; . Genevestigator P38489; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0004155; F:6; 7-dihydropteridine reductase activity; IEA:EC InterPro IPR000415; Nitroreductase-like; . Gene3D G3DSA:3.40.109.10; G3DSA:3.40.109.10; 1; . Pfam PF00881; Nitroreductase; 1; . SUPFAM SSF55469; Nitroreductase; 1; . SWISS-2DPAGE (search AC)