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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P38489




SWISS-2DPAGE:  P38489


P38489


General information about the entry
View entry in simple text format
Entry nameNFNB_ECOLI
Primary accession numberP38489
integrated into SWISS-2DPAGE on October 1, 1998 (release 8)
2D Annotations were last modified onMarch 31, 2004 (version 4)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase;.
Gene nameName=nfnB
Synonyms=dprA, nfsB, nfsI, ntr
OrderedLocusNames=b0578, JW0567
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.55; Mw=25092  [identification data]
pI=5.09; Mw=14160  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.47; Mw=32502  [identification data]
pI=5.32; Mw=28018  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.83; Mw=24964  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP38489; NFNB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameNFNB_ECOLI
Primary accession numberP38489
Secondary accession number(s) P19575
Sequence was last modified on October 1, 1994 (version 1)
Annotations were last modified on October 19, 2011 (version 101)
Name and origin of the protein
DescriptionRecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase;
Gene nameName=nfnB
Synonyms=dprA, nfsB, nfsI, ntr
OrderedLocusNames=b0578, JW0567
Encoded onName=nfnB; Synonyms=dprA, nfsB, nfsI, ntr; OrderedLocusNames=b0578, JW0567
Keywords3D-structure; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; NAD; NADP; Oxidoreductase; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD25414; BAA05004.1; -; Genomic_DNA
EMBLU07860; AAC43263.1; -; Genomic_DNA
EMBLU82598; AAB40776.1; -; Genomic_DNA
EMBLU00096; AAC73679.1; -; Genomic_DNA
EMBLAP009048; BAA35218.1; -; Genomic_DNA
PIRI67685; I67685; .
PIRS01818; S01818; .
RefSeqNP_415110.1; NC_000913.2; .
PDB1DS7; X-ray; 2.06 A; A/B=1-217
PDB1ICR; X-ray; 1.70 A; A/B=1-217
PDB1ICU; X-ray; 1.80 A; A/B/C/D=1-217
PDB1ICV; X-ray; 2.40 A; A/B/C/D=1-217
PDB1IDT; X-ray; 2.00 A; A/B=1-217
PDB1OO5; X-ray; 2.50 A; A/B=1-217
PDB1OO6; X-ray; 2.00 A; A/B=1-217
PDB1OON; X-ray; 2.49 A; A/B=1-217
PDB1OOQ; X-ray; 2.00 A; A/B=1-217
PDB1YKI; X-ray; 1.70 A; A/B/C/D=1-217
PDB1YLR; X-ray; 1.70 A; A/B=1-217
PDB1YLU; X-ray; 2.00 A; A/B=1-217
PDBsum1DS7; -; .
PDBsum1ICR; -; .
PDBsum1ICU; -; .
PDBsum1ICV; -; .
PDBsum1IDT; -; .
PDBsum1OO5; -; .
PDBsum1OO6; -; .
PDBsum1OON; -; .
PDBsum1OOQ; -; .
PDBsum1YKI; -; .
PDBsum1YLR; -; .
PDBsum1YLU; -; .
ProteinModelPortalP38489; -; .
SMRP38489; 1-217; .
DIPDIP-10330N; -; .
IntActP38489; 12; .
SWISS-2DPAGEP38489; -; .
EnsemblBacteriaEBESCT00000003087; EBESCP00000003087; EBESCG00000002529; .
EnsemblBacteriaEBESCT00000014539; EBESCP00000013830; EBESCG00000013600; .
GeneID945778; -; .
GenomeReviewsAP009048_GR; JW0567; .
GenomeReviewsU00096_GR; b0578; .
KEGGecj:JW0567; -; .
KEGGeco:b0578; -; .
EchoBASEEB4146; -; .
EcoGeneEG20151; nfnB; .
eggNOGCOG0778; -; .
GeneTreeEBGT00050000010591; -; .
HOGENOMHBG751302; -; .
OMAKRYSTKA; -; .
ProtClustDBPRK11053; -; .
BioCycEcoCyc:DIHYDROPTERIREDUCT-MONOMER; -; .
BioCycMetaCyc:DIHYDROPTERIREDUCT-MONOMER; -; .
DrugBankDB01093; Dimethyl sulfoxide; .
GenevestigatorP38489; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0004155; F:6; 7-dihydropteridine reductase activity; IEA:EC
InterProIPR000415; Nitroreductase-like; .
Gene3DG3DSA:3.40.109.10; G3DSA:3.40.109.10; 1; .
PfamPF00881; Nitroreductase; 1; .
SUPFAMSSF55469; Nitroreductase; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server