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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P38489 |
SWISS-2DPAGE: P38489
P38489:
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ID NFNB_ECOLI; STANDARD; 2DG.
AC P38489;
DT 01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT 31-MAR-2004, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-;
DE AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName:
DE Full=FMN-dependent nitroreductase;.
GN Name=nfnB; Synonyms=dprA, nfsB, nfsI, ntr; OrderedLocusNames=b0578, JW0567;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-001HEN=5.55/25092;
2D -!- PI/MW: SPOT 2D-0015EU=5.09/14160;
2D -!- PEPTIDE MASSES: SPOT 2D-001HEN: 939.358; 1005.623; 1156.665;
2D 1216.618; 1227.602; 2566.352; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0015EU: 1179.595; 1227.587; 1355.701;
2D 1370.802; 1961.013; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LGL=5.47/32502;
2D -!- PI/MW: SPOT 2D-001LKH=5.32/28018;
2D -!- PEPTIDE MASSES: SPOT 2D-001LGL: 923.475; 939.455; 1051.573;
2D 1067.54; 1216.623; 1227.626; 1284.743; 1330.629; 1370.822; 2566.264; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001LKH: 923.425; 939.403; 1051.53; 1067.51;
2D 1094.537; 1156.626; 1216.592; 1227.59; 1284.718; 1330.603; 1346.59;
2D 1370.783; 1458.661; 1492.773; 2566.285; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WU3=5.83/24964;
2D -!- PEPTIDE MASSES: SPOT 2D-001WU3: 1156.52; 1216.5; 1227.5; 1284.62;
2D 1355.61; 1370.72; 1960.96; 2566.28; 2823.41; 2907.38; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WU3:
2D (K)TLLQYSPSSTNSQPWHFIVASTEEGK(A),32-57; (K)SAAGNYVFNERK(M),63-74;
2D (K)LVVDQEDADGR(F),97-107; (K)LVVDQEDADGRFATPEAK(A),97-114.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [2].
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DR UniProtKB/Swiss-Prot; P38489; NFNB_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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