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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P61714 |
SWISS-2DPAGE: P61714
P61714:
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ID RISB_ECOLI; STANDARD; 2DG.
AC P61714; P25540;
DT 01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT 30-DEC-2004, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=6,7-dimethyl-8-ribityllumazine synthase; Short=DMRL
DE synthase; Short=Lumazine synthase; EC=2.5.1.9; AltName: Full=Riboflavin
DE synthase beta chain;.
GN Name=ribH; Synonyms=ribE, ybaF; OrderedLocusNames=b0415, JW0405;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015FE=5.19/12934;
2D -!- PEPTIDE MASSES: SPOT 2D-0015FE: 743.421; 1290.71; 1530.72; 1576.84;
2D 2150.03; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1], and microsequencing
2D (MNIIE) [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WXK=5.15/16000;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WXK: (R)FNNFINDSLLEGAIDALKR,22-40;
2D (R)IGQVKDENITVVWVPGAYELPLAAGALAK,41-69; (K)TGKYDAVIALGTVIR,70-84.
2D -!- EXPRESSION: decrease after benzoic acid treatment [2].
2D -!- MAPPING: Tandem mass spectrometry [2].
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DR UniProtKB/Swiss-Prot; P61714; RISB_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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