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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P61889




SWISS-2DPAGE:  P61889


P61889


General information about the entry
View entry in simple text format
Entry nameMDH_ECOLI
Primary accession numberP61889
Secondary accession number(s) P06994
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onDecember 30, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase; EC=1.1.1.37;.
Gene nameName=mdh
OrderedLocusNames=b3236, JW3205
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.55; Mw=35602
pI=5.43; Mw=35182  [identification data]
pI=5.62; Mw=33398

MAPPING (identification):
SPOTS 2D-000L61, 2D-000L82: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
SPOT 2D-000L6K: AMINO ACID COMPOSITION AND SEQUENCE TAG (VA) [3].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.57; Mw=40903  [identification data]
pI=5.49; Mw=38144  [identification data]
pI=5.25; Mw=37049  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=6.86; Mw=29566  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.56; Mw=35156  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP61889; MDH_ECOLI.
ECO2DBASEF030.2; 6TH EDITION.
UniProtKB/Swiss-ProtP61889; MDH_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMDH_ECOLI
Primary accession numberP61889
Secondary accession number(s) O30401 O30402 O30403 P06994 Q2M8X7 Q59343 Q59344 Q59345 Q59346 Q59347 Q59348 Q60133 Q60150 Q933J3 Q93R02 Q9FDQ3 Q9FDQ4 Q9FDQ5
Sequence was last modified on June 7, 2004 (version 1)
Annotations were last modified on October 19, 2011 (version 78)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase; EC=1.1.1.37;
Gene nameName=mdh
OrderedLocusNames=b3236, JW3205
Encoded onName=mdh; OrderedLocusNames=b3236, JW3205
Keywords3D-structure; Complete proteome; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY00129; CAA68326.1; -; Genomic_DNA
EMBLM24777; AAA16107.1; -; Unassigned_DNA
EMBLU18997; AAA58038.1; -; Genomic_DNA
EMBLU00096; AAC76268.1; -; Genomic_DNA
EMBLAP009048; BAE77279.1; -; Genomic_DNA
EMBLM10417; AAA24147.1; -; Genomic_DNA
EMBLU04742; AAC43730.1; -; Genomic_DNA
EMBLU04743; AAC43731.1; -; Genomic_DNA
EMBLU04744; AAC43732.1; -; Genomic_DNA
EMBLU04745; AAC43733.1; -; Genomic_DNA
EMBLU04746; AAC43734.1; -; Genomic_DNA
EMBLU04747; AAC43735.1; -; Genomic_DNA
EMBLU04748; AAC43736.1; -; Genomic_DNA
EMBLU04749; AAC43737.1; -; Genomic_DNA
EMBLU04750; AAC43738.1; -; Genomic_DNA
EMBLU04751; AAC43739.1; -; Genomic_DNA
EMBLU04752; AAC43740.1; -; Genomic_DNA
EMBLU04753; AAC43741.1; -; Genomic_DNA
EMBLU04754; AAC43742.1; -; Genomic_DNA
EMBLU04755; AAC43743.1; -; Genomic_DNA
EMBLU04756; AAC43744.1; -; Genomic_DNA
EMBLU04757; AAC43745.1; -; Genomic_DNA
EMBLU04758; AAC43746.1; -; Genomic_DNA
EMBLU04759; AAC43747.1; -; Genomic_DNA
EMBLU04760; AAC43748.1; -; Genomic_DNA
EMBLU04770; AAC43758.1; -; Genomic_DNA
EMBLAF004170; AAB87003.1; -; Genomic_DNA
EMBLAF004171; AAB87004.1; -; Genomic_DNA
EMBLAF004172; AAB87005.1; -; Genomic_DNA
EMBLAF004173; AAB87006.1; -; Genomic_DNA
EMBLAF004174; AAB87007.1; -; Genomic_DNA
EMBLAF004175; AAB87008.1; -; Genomic_DNA
EMBLAF004176; AAB87009.1; -; Genomic_DNA
EMBLAF004177; AAB87010.1; -; Genomic_DNA
EMBLAF004179; AAB87012.1; -; Genomic_DNA
EMBLAF004180; AAB87013.1; -; Genomic_DNA
EMBLAF004182; AAB87015.1; -; Genomic_DNA
EMBLAF004183; AAB87016.1; -; Genomic_DNA
EMBLAF004184; AAB87017.1; -; Genomic_DNA
EMBLAF004186; AAB87019.1; -; Genomic_DNA
EMBLAF004187; AAB87020.1; -; Genomic_DNA
EMBLAF004188; AAB87021.1; -; Genomic_DNA
EMBLAF004190; AAB87023.1; -; Genomic_DNA
EMBLAF004191; AAB87024.1; -; Genomic_DNA
EMBLAF004195; AAB87028.1; -; Genomic_DNA
EMBLAF004196; AAB87029.1; -; Genomic_DNA
EMBLAF004199; AAB87032.1; -; Genomic_DNA
EMBLAF004200; AAB87033.1; -; Genomic_DNA
EMBLAF004201; AAB87034.1; -; Genomic_DNA
EMBLAF004202; AAB87035.1; -; Genomic_DNA
EMBLAF004203; AAB87036.1; -; Genomic_DNA
EMBLAF004204; AAB87037.1; -; Genomic_DNA
EMBLAF004205; AAB87038.1; -; Genomic_DNA
EMBLAF004206; AAB87039.1; -; Genomic_DNA
EMBLAF004207; AAB87040.1; -; Genomic_DNA
EMBLAF004208; AAB87041.1; -; Genomic_DNA
EMBLAF004209; AAB87042.1; -; Genomic_DNA
EMBLAF091758; AAF97988.1; -; Genomic_DNA
EMBLAF091759; AAF97989.1; -; Genomic_DNA
EMBLAF091760; AAF97990.1; -; Genomic_DNA
EMBLAF091761; AAF97991.1; -; Genomic_DNA
EMBLAF091762; AAF97992.1; -; Genomic_DNA
EMBLAF091763; AAF97993.1; -; Genomic_DNA
EMBLAF091764; AAF97994.1; -; Genomic_DNA
EMBLAF091765; AAF97995.1; -; Genomic_DNA
EMBLAF091766; AAF97996.1; -; Genomic_DNA
EMBLAF091767; AAF97997.1; -; Genomic_DNA
EMBLAF091768; AAF97998.1; -; Genomic_DNA
EMBLAF091769; AAF97999.1; -; Genomic_DNA
EMBLAF091770; AAF98000.1; -; Genomic_DNA
EMBLAF091771; AAF98001.1; -; Genomic_DNA
EMBLAF091772; AAF98002.1; -; Genomic_DNA
EMBLAF091773; AAF98003.1; -; Genomic_DNA
EMBLAF091774; AAF98004.1; -; Genomic_DNA
EMBLAF091775; AAF98005.1; -; Genomic_DNA
EMBLAF091776; AAF98006.1; -; Genomic_DNA
EMBLAF091777; AAF98007.1; -; Genomic_DNA
EMBLAF091778; AAF98008.1; -; Genomic_DNA
PIRF65115; DEECM; .
RefSeqNP_417703.1; NC_000913.2; .
PDB1CME; Model; -; A=1-312
PDB1EMD; X-ray; 1.90 A; A=1-312
PDB1IB6; X-ray; 2.10 A; A/B/C/D=1-312
PDB1IE3; X-ray; 2.50 A; A/B/C/D=1-312
PDB2CMD; X-ray; 1.87 A; A=1-312
PDB2PWZ; X-ray; 2.20 A; A/C/E/G=1-312
PDB3HHP; X-ray; 1.45 A; A/B/C/D=1-312
PDBsum1CME; -; .
PDBsum1EMD; -; .
PDBsum1IB6; -; .
PDBsum1IE3; -; .
PDBsum2CMD; -; .
PDBsum2PWZ; -; .
PDBsum3HHP; -; .
ProteinModelPortalP61889; -; .
SMRP61889; 1-312; .
DIPDIP-35924N; -; .
IntActP61889; 8; .
PhosSiteP61889; -; .
SWISS-2DPAGEP61889; -; .
2DBase-EcoliP61889; -; .
ECO2DBASEF030.2; 6TH EDITION; .
PRIDEP61889; -; .
EnsemblBacteriaEBESCT00000004820; EBESCP00000004820; EBESCG00000003936; .
EnsemblBacteriaEBESCT00000017497; EBESCP00000016788; EBESCG00000016553; .
GeneID947854; -; .
GenomeReviewsAP009048_GR; JW3205; .
GenomeReviewsU00096_GR; b3236; .
KEGGecj:JW3205; -; .
KEGGeco:b3236; -; .
EchoBASEEB0571; -; .
EcoGeneEG10576; mdh; .
GeneTreeEBGT00050000011757; -; .
OMALVEKCAA; -; .
ProtClustDBPRK05086; -; .
BioCycEcoCyc:MALATE-DEHASE-MONOMER; -; .
BioCycMetaCyc:MALATE-DEHASE-MONOMER; -; .
DrugBankDB00336; Nitrofurazone; .
GenevestigatorP61889; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0000300; C:peripheral to membrane of membrane fraction; IDA:EcoliWiki; .
GOGO:0005488; F:binding; IEA:InterPro; .
GOGO:0030060; F:L-malate dehydrogenase activity; IEA:EC; .
GOGO:0009061; P:anaerobic respiration; IDA:EcoliWiki; .
GOGO:0006113; P:fermentation; IDA:EcoliWiki; .
GOGO:0006096; P:glycolysis; IDA:EcoliWiki; .
GOGO:0006108; P:malate metabolic process; IDA:EcoliWiki; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:EcoliWiki; .
HAMAPMF_01516; Malate_dehydrog_1; 1; -
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR010097; Malate_DH_type1; .
InterProIPR023958; Malate_DH_type1_bac; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01772; MDH_euk_gproteo; 1; .
PROSITEPS00068; MDH; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server