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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P61889




SWISS-2DPAGE: P61889



P61889:

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ID   MDH_ECOLI; STANDARD; 2DG.
AC   P61889; P06994;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   30-DEC-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=Malate dehydrogenase; EC=1.1.1.37;.
GN   Name=mdh; OrderedLocusNames=b3236, JW3205;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMODIMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L61=5.55/35602;
2D   -!-   PI/MW: SPOT 2D-000L6K=5.43/35182;
2D   -!-   PI/MW: SPOT 2D-000L82=5.62/33398;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000L6K: B=5.9, Z=9.5, S=6.2, H=0.2,
2D         G=11.9, T=6.2, A=11.4, P=5.2, Y=1.7, R=3.7, V=10.2, M=0.9, I=5.4, L=10.9,
2D         F=5.1, K=5.8;
2D   -!-   MAPPING: SPOTS 2D-000L61, 2D-000L82: AMINO ACID COMPOSITION AND
2D         MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
2D         SPOT 2D-000L6K: AMINO ACID COMPOSITION AND SEQUENCE TAG (VA) [3].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LA5=5.57/40903;
2D   -!-   PI/MW: SPOT 2D-001LC6=5.49/38144;
2D   -!-   PI/MW: SPOT 2D-001LD3=5.25/37049;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LA5: 899.631; 1008.641; 1149.793;
2D         1193.776; 1234.771; 1247.881; 1276.824; 1405.839; 1414.923; 1421.853;
2D         1735.262; 2366.454; 2399.35; 2402.428; 2643.668; 3376.123; 4312.703; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LC6: 899.183; 1008.15; 1149.214;
2D         1193.177; 1234.166; 1247.292; 1276.205; 1414.244; 1421.138; 1734.384; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LD3: 899.676; 1008.707; 1149.87;
2D         1193.867; 1247.996; 1276.926; 1405.977; 1415.043; 1421.945; 1735.358;
2D         2399.536; 2402.648; 2643.872; 3376.377; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI6-11;
2D   -!-   PI/MW: SPOT 2D-001TNT=6.86/29566;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001TNT: 899.407; 1008.438; 1193.499;
2D         1234.582; 1247.641; 1276.551; 1734.911; 2402.031; 3375.496; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WOZ=5.56/35156;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WOZ: 1008.56; 1149.69; 1234.67; 1247.77;
2D         1258.69; 1276.73; 1421.75; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
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DR   2DBase-Ecoli; P61889; MDH_ECOLI.
DR   ECO2DBASE; F030.2; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P61889; MDH_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli(6-11)
ECOLI6-11
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server