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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P68066




SWISS-2DPAGE: P68066



P68066:

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ID   GRCA_ECOLI; STANDARD; 2DG.
AC   P68066; P33633;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   01-OCT-2001, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Autonomous glycyl radical cofactor;.
GN   Name=grcA; Synonyms=yfiD; OrderedLocusNames=b2579, JW2563;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI4.5-5.5.
IM   ECOLI, ECOLI4-5, ECOLI4.5-5.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LSC=5.01/15083;
2D   -!-   PI/MW: SPOT 2D-000LTS=5.00/12496;
2D   -!-   PI/MW: SPOT 2D-000LUC=5.13/11775;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000LSC: B=8.6, Z=13.5, S=5.6,
2D         H=0.5, G=8.3, T=5.2, A=9.3, P=4.7, Y=2.2, R=7.8, V=11.5, M=0.7, I=5.1,
2D         L=9.3, F=2.9, K=4.7;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000LUC: B=6.3, Z=14.8, S=5.1,
2D         H=0.3, G=9.1, T=3.6, A=10, P=4.7, Y=1.9, R=6.8, V=13.3, M=1.2, I=5.4,
2D         L=11, F=1.5, K=5.1;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (MITG) [1].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HON=5.01/11128; !FRAGMENT!
2D   -!-   PI/MW: SPOT 2D-001HOT=4.96/10675;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HON: 890.475; 1219.58; 1280.686;
2D         1381.727; 1434.77; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HOT: 865.46; 890.571; 1219.673;
2D         1280.782; 1381.807; 1434.842; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0015FR=5.09/11594;
2D   -!-   PI/MW: SPOT 2D-0015GW=5.05/9918;
2D   -!-   PI/MW: SPOT 2D-0015H1=5.23/9795;
2D   -!-   PI/MW: SPOT 2D-0015H7=5.21/9583;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0015FR: 751.415; 1219.59; 1280.7; 1381.76;
2D         1434.75; 1590.85; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0015H1: 890.496; 1280.677; 1434.771; TRYPSIN.
2D   -!-   MAPPING: SPOTS 15FR, 15H1: Peptide mass fingerprinting [2]; SPOTS
2D         15GW, 15H7: MICROSEQUENCING (MITGI) [2].
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DR   UniProtKB/Swiss-Prot; P68066; GRCA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server