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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P99041 |
SWISS-2DPAGE: P99041
P99041:
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ID ACTB_MOUSE; PRELIMINARY; 2DG.
AC P99041; P99020; P99021; P99022;
DT 01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT 01-OCT-2001, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 8.
DE RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains:
DE RecName: Full=Actin, cytoplasmic 1, N-terminally processed;.
GN Name=Actb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
MT BAT_MOUSE, ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, NUCLEI_LIVER_MOUSE, WAT_MOUSE.
IM BAT_MOUSE, ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, NUCLEI_LIVER_MOUSE, WAT_MOUSE.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96154592; PubMed=8586073; [NCBI, Expasy, EBI, Israel, Japan]
RA Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers
RA J., Steiner S., Witzmann F., Anderson N.G.;
RT ''''''An updated two-dimensional gel database of rat liver proteins
RT useful in gene regulation and drug effect studies'';'';'';
RL Electrophoresis 16(1):1977-1981(1995).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=93162044; PubMed=1286668; [NCBI, Expasy, EBI, Israel, Japan]
RA Giometti C.S., Taylor J., Tollaksen S.L.;
RT ''''''Mouse liver protein database: a catalog of proteins detected by
RT two- dimensional gel electrophoresis'';'';'';
RL Electrophoresis 13(1):970-991(1992).
RN [3]
RP MAPPING ON GEL.
RX PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
RA Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano
RA S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A.,
RA Hochstrasser D.F.;
RT ''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of
RT diabetes and obesity'';'';'';
RL Proteomics 1(1):136-163(2001).
2D -!- MASTER: BAT_MOUSE;
2D -!- PI/MW: SPOT 2D-001PWO=5.10/40780;
2D -!- PI/MW: SPOT 2D-001PWQ=5.17/40780;
2D -!- PI/MW: SPOT 2D-001PWR=5.21/40780;
2D -!- PI/MW: SPOT 2D-001PWS=5.25/40780;
2D -!- PI/MW: SPOT 2D-001PWT=5.27/40880;
2D -!- PI/MW: SPOT 2D-001PWX=5.02/40780;
2D -!- PI/MW: SPOT 2D-001PWY=5.29/40780;
2D -!- PI/MW: SPOT 2D-001PX7=4.96/40730;
2D -!- PI/MW: SPOT 2D-001PX8=5.28/40730;
2D -!- MAPPING: GEL MATCHING WITH WAT_MOUSE [3].
2D -!- MASTER: ISLETS_MOUSE;
2D -!- PI/MW: SPOT 2D-0018NU=5.12/48613;
2D -!- PI/MW: SPOT 2D-0018OC=5.16/48747;
2D -!- PI/MW: SPOT 2D-0018RG=5.17/44382;
2D -!- PI/MW: SPOT 2D-0018RM=5.21/44505;
2D -!- PEPTIDE MASSES: SPOT 2D-0018NU: 976.417; 1132.527; 1198.685;
2D 1515.749; 1516.677; 1790.885; 1954.056; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0018OC: 976.526; 1132.604; 1198.76;
2D 1515.809; 1516.745; 1790.955; 1954.091; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0018RG: 976.474; 1130.55; 1500.689;
2D 1790.798; 1955.942; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0018RM: 976.457; 998.419; 1132.547;
2D 1516.727; 1790.915; 1954.074; 2231.084; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3].
2D -!- MASTER: LIVER_MOUSE;
2D -!- PI/MW: SPOT 2D-0013LF=5.15/44157;
2D -!- PI/MW: SPOT 2D-0013NO=5.26/41907;
2D -!- PI/MW: SPOT 2D-0013NP=5.27/42117;
2D -!- PEPTIDE MASSES: SPOT 2D-0013LF: 1132.456; 1198.649; 1516.718;
2D 1790.835; TRYPSIN.
2D -!- MAPPING: SPOT 2D-0013LF: Peptide mass fingerprinting [3]; SPOTS
2D 2D-0013N*: MATCHING [3] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1],
2D AND WITH GIOMETTI MOUSE LIVER 2-DE MAP [2].
2D -!- MASTER: MUSCLE_MOUSE;
2D -!- PI/MW: SPOT 2D-0016WE=5.20/50527;
2D -!- PI/MW: SPOT 2D-0016XE=5.19/47815;
2D -!- PI/MW: SPOT 2D-0016ZF=5.20/44017;
2D -!- PI/MW: SPOT 2D-001711=5.13/43175;
2D -!- PI/MW: SPOT 2D-001717=5.06/43414;
2D -!- PI/MW: SPOT 2D-00171G=5.27/42937;
2D -!- PI/MW: SPOT 2D-001732=5.09/41351;
2D -!- PEPTIDE MASSES: SPOT 2D-0016ZF: 976.458; 1130.537; 1198.678;
2D 1500.669; 1515.714; 1790.835; 1955.947; 2261.934; 2277.919; 3212.101; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001711: 1500.512; 1515.555; 1790.794;
2D 1955.987; 2278.073; 3212.722; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001717: 976.448; 1130.552; 1203.551;
2D 1500.716; 1515.753; 1790.909; 1956.045; 2278.024; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-00171G: 976.422; 1130.556; 1500.718;
2D 1515.76; 1790.91; 1956.029; 2277.98; 2367.299; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001732: 976.423; 1130.543; 1515.766;
2D 1790.9; 1956.058; 2367.263; TRYPSIN.
2D -!- MAPPING: SPOTS 2D-0016*, 2D-0017*: MATCHING [3] WITH THE MOUSE
2D CARDIAC 2-DE GEL [3]; SPOTS 2D-0016ZF, 2D-0017*: MASS FINGERPRINTING [3].
2D -!- MASTER: NUCLEI_LIVER_MOUSE;
2D -!- PI/MW: SPOT 2D-001NO7=5.24/41605;
2D -!- PI/MW: SPOT 2D-001NOK=5.14/41718;
2D -!- PI/MW: SPOT 2D-001NOW=5.05/41946;
2D -!- PI/MW: SPOT 2D-001NP9=5.19/41504;
2D -!- PI/MW: SPOT 2D-001O84=4.57/27449; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001NO7: 976.454; 1132.518; 1198.713;
2D 1354.644; 1790.902; 1954.078; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001NOK: 976.428; 1198.704; 1354.634;
2D 1790.859; 1954.073; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001NOW: 976.458; 1132.495; 1198.726;
2D 1516.704; 1790.793; 1953.927; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001NP9: 976.482; 1130.557; 1198.723;
2D 1790.854; 1955.985; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001O84: 976.486; 1198.753; 1790.917;
2D 1956.043; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3].
2D -!- MASTER: WAT_MOUSE;
2D -!- PI/MW: SPOT 2D-001BBI=5.27/42000;
2D -!- PI/MW: SPOT 2D-001BBU=5.23/42000;
2D -!- PI/MW: SPOT 2D-001BBV=5.26/41845;
2D -!- PI/MW: SPOT 2D-001BC5=5.18/42132;
2D -!- PEPTIDE MASSES: SPOT 2D-001BBI: 1132.614; 1515.789; 1516.754;
2D 1610.778; 1790.833; 1954.032; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001BBV: 976.441; 1132.533; 1515.758;
2D 1516.732; 1790.896; 1954.051; 2231.113; 2367.247; TRYPSIN.
2D -!- MAPPING: MATCHING WITH THE MOUSE LIVER MASTER GEL [3]; SPOTS 1BBI,
2D 1BBV: Peptide mass fingerprinting [3].
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DR COMPLUYEAST-2DPAGE; P60710; ACTB_MOUSE.
DR REPRODUCTION-2DPAGE; IPI00110850; IPI00110850.
DR REPRODUCTION-2DPAGE; P60710; P60710.
DR UCD-2DPAGE; P60710; ACTB_MOUSE.
DR UniProtKB/Swiss-Prot; P60710; ACTB_MOUSE.
//
The following 2-D maps are available for this protein:
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Brown adipose tissue
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Pancreatic islet cells
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Liver
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Gastrocnemius muscle
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Soluble nuclear proteins and matrix from liver tissue
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White adipose tissue
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SWISS-2DPAGE (search AC)
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