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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P99056




SWISS-2DPAGE:  P99056


P99056


General information about the entry
View entry in simple text format
Entry nameMETK1_ARATH
Primary accession numberP99056
integrated into SWISS-2DPAGE on December 1, 2000 (release 13)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=S-adenosylmethionine synthase 1; Short=AdoMet synthase 1; EC=2.5.1.6; AltName: Full=Methionine adenosyltransferase 1; Short=MAT 1;.
Gene nameName=SAM1
OrderedLocusNames=At1g02500
ORFNames=T14P4.17, T14P4_22
Annotated speciesArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
TaxonomyEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core; eudicotyledons; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
References
[1]   MAPPING ON GEL
Sarazin B., Tonella L., Marques K., Paesano S., Chane-Favre L., Sanchez J.-C., Hochstrasser D.F., Thiellement H.
Submitted (OCT-2000) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

ARABIDOPSIS {Arabidopsis thaliana}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
ARABIDOPSIS
  map experimental info
  protein estimated location
 
ARABIDOPSIS

MAP LOCATIONS:
pI=4.96; Mw=45509  [identification data]

MAPPING (identification):
MASS SPECTROMETRY [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP23686; METK1_ARATH.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMETK1_ARATH
Primary accession numberP23686
Secondary accession number(s) Q941A8 Q9FEE0
Sequence was last modified on June 20, 2002 (version 2)
Annotations were last modified on October 19, 2011 (version 115)
Name and origin of the protein
DescriptionRecName: Full=S-adenosylmethionine synthase 1; Short=AdoMet synthase 1; EC=2.5.1.6; AltName: Full=Methionine adenosyltransferase 1; Short=MAT 1;
Gene nameName=SAM1
OrderedLocusNames=At1g02500
ORFNames=T14P4.17, T14P4_22
Encoded onName=SAM1; OrderedLocusNames=At1g02500; ORFNames=T14P4.17, T14P4_22
KeywordsATP-binding; Cobalt; Complete proteome; Cytoplasm; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; S-nitrosylation; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM55077; AAA32868.1; -; Genomic_DNA
EMBLAC022521; AAG10639.1; -; Genomic_DNA
EMBLCP002684; AEE27437.1; -; Genomic_DNA
EMBLCP002684; AEE27438.1; -; Genomic_DNA
EMBLAF325061; AAG40413.1; -; mRNA
EMBLAY092955; AAM12954.1; -; mRNA
EMBLAF428440; AAL16209.1; -; mRNA
EMBLAY052311; AAK96504.1; ALT_FRAME; mRNA
EMBLAY061895; AAL31222.1; ALT_FRAME; mRNA
EMBLAY087703; AAM65240.1; -; mRNA
EMBLZ17672; CAA79031.1; -; mRNA
EMBLZ17762; CAA79057.1; -; mRNA
IPIIPI00545903; -; .
PIRC86155; C86155; .
PIRJN0131; JN0131; .
RefSeqNP_171751.1; NM_100131.2; .
RefSeqNP_849577.1; NM_179246.1; .
UniGeneAt.24731; -; .
UniGeneAt.72241; -; .
ProteinModelPortalP23686; -; .
SMRP23686; 3-388; .
STRINGP23686; -; .
SWISS-2DPAGEP99056; -; .
PRIDEP23686; -; .
ProMEXP23686; -; .
EnsemblPlantsAT1G02500.1; AT1G02500.1; AT1G02500; .
EnsemblPlantsAT1G02500.2; AT1G02500.2; AT1G02500; .
GeneID839501; -; .
GenomeReviewsCT485782_GR; AT1G02500; .
KEGGath:AT1G02500; -; .
NMPDRfig|3702.1.peg.370; -; .
TAIRAt1g02500; -; .
eggNOGKOG1506; -; .
GeneTreeEPGT00050000019410; -; .
HOGENOMHBG443662; -; .
InParanoidP23686; -; .
OMASTKGFDY; -; .
PhylomeDBP23686; -; .
ProtClustDBPLN02243; -; .
BioCycARA:AT1G02500-MONOMER; -; .
BioCycMetaCyc:AT1G02500-MONOMER; -; .
ArrayExpressP23686; -; .
GenevestigatorP23686; -; .
GermOnlineAT1G02500; Arabidopsis thaliana; .
GOGO:0005618; C:cell wall; IDA:TAIR; .
GOGO:0005829; C:cytosol; IDA:TAIR; .
GOGO:0005886; C:plasma membrane; IDA:TAIR; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004478; F:methionine adenosyltransferase activity; IDA:TAIR; .
GOGO:0071281; P:cellular response to iron ion; IEP:TAIR; .
GOGO:0009693; P:ethylene biosynthetic process; TAS:TAIR; .
GOGO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW; .
GOGO:0046686; P:response to cadmium ion; IEP:TAIR; .
GOGO:0009651; P:response to salt stress; IEP:TAIR; .
GOGO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:InterPro; .
InterProIPR022631; ADOMET_SYNTHASE_CS; .
InterProIPR022630; S-AdoMet_synt_C; .
InterProIPR022629; S-AdoMet_synt_central; .
InterProIPR022628; S-AdoMet_synt_N; .
InterProIPR002133; S-AdoMet_synthetase; .
InterProIPR022636; S-AdoMet_synthetase_sfam; .
PANTHERPTHR11964; S-AdoMet_synt; 1; .
PfamPF02773; S-AdoMet_synt_C; 1; .
PfamPF02772; S-AdoMet_synt_M; 1; .
PfamPF00438; S-AdoMet_synt_N; 1; .
PIRSFPIRSF000497; MAT; 1; .
SUPFAMSSF55973; S-AdoMet_synt; 3; .
TIGRFAMsTIGR01034; MetK; 1; .
PROSITEPS00376; ADOMET_SYNTHASE_1; 1; .
PROSITEPS00377; ADOMET_SYNTHASE_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server