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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PDIA1_HUMAN




SWISS-2DPAGE: PDIA1_HUMAN



PDIA1_HUMAN:

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ID   PDIA1_HUMAN; STANDARD; 2DG.
AC   P07237; P30037; P32079;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1;
DE   AltName: Full=Cellular thyroid hormone-binding protein; AltName:
DE   Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor;.
GN   Name=P4HB; Synonyms=ERBA2L, PDI, PDIA1, PO4DB;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
MT   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
IM   DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
IM   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [5]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [6]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [7]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [8]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001DTH=4.77/54763;
2D   -!-   PI/MW: SPOT 2D-001DTP=4.80/54203;
2D   -!-   PI/MW: SPOT 2D-001DU1=4.84/54064;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001DTP: 910.467; 966.572; 1066.54; 1081.67;
2D         1521.76; 1780.83; 1833.9; 1965.03; TRYPSIN.
2D   -!-   MAPPING: GEL MATCHING WITH HEPG2 MASTER GEL AND MASS FINGERPRINTING [7].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000E4L=4.76/57697;
2D   -!-   PI/MW: SPOT 2D-000E4M=4.80/57500;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0002NM=4.71/55308;
2D   -!-   PI/MW: SPOT 2D-0002NN=4.74/55308;
2D   -!-   PI/MW: SPOT 2D-0002NR=4.68/55462;
2D   -!-   PI/MW: SPOT 2D-0002NW=4.79/55308;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000YNF=4.73/56100;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [5][6].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000MV6=4.76/54532;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000MV6: B=13.2, Z=16.1, S=4.9,
2D         H=1.5, G=7.5, T=4.6, A=9.9, P=4.3, Y=4.1, R=2.9, V=5.4, M=0.7, I=3.9,
2D         L=8, F=5.2, K=7.7;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID
2D         COMPOSITION ANALYSIS [4].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0000MF=4.76/54846;
2D   -!-   PI/MW: SPOT 2D-0000MG=4.82/54695;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001XZF=4.65/43614;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001XZF: 870.43532; 905.4054; 910.4519;
2D         1066.4867; 1451.7173; 1459.7071; 1521.7765; 1780.8465; 1833.9008; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [8].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0007H6=4.74/54509;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0005ZK=4.90/51909;
2D   -!-   MAPPING: MICROSEQUENCING [3].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000CSH=4.81/54949;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC   ---------------------------------------------------------------------------
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CC   its content is in no way modified and this statement is not removed.
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DR   DOSAC-COBS 2D-PAGE; P07237; P07237.
DR   DOSAC-COBS-2DPAGE; P07237; PDIA1_HUMAN.
DR   OGP-WWW; P07237.
DR   REPRODUCTION-2DPAGE; IPI00010796; IPI00010796.
DR   REPRODUCTION-2DPAGE; P07237; P07237.
DR   Siena-2DPAGE; P07237; PDIA1_HUMAN.
DR   UCD-2DPAGE; P07237; PDIA1_HUMAN.
DR   UniProtKB/Swiss-Prot; P07237; PDIA1_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2
HEPG2_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Liver
LIVER_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Lymphoma
LYMPHOMA_HUMAN
Red blood cells
RBC_HUMAN
Macrophage Like Cell Line
U937_HUMAN
  


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server