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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: PFLB_ECOLI SWISS-2DPAGE:  PFLB_ECOLI PFLB_ECOLI General information about the entry View entry in simple text format Entry name PFLB_ECOLI Primary accession number P09373 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on October 1, 2001 (version 1) General Annotations were last modified on  May 19, 2011 (version 11) Name and origin of the protein Description RecName: Full=Formate acetyltransferase 1; EC=2.3.1.54; AltName: Full=Pyruvate formate-lyase 1;. Gene name Name=pflB Synonyms=pfl OrderedLocusNames=b0903, JW0886 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan] Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L. ''''''Gene-protein index of Escherichia coli K-12'';'';'' Microbiol. Rev. 47(1):231-284(1983) [3]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) Comments SUBUNIT: HOMODIMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000KFK: pI=5.61; Mw=85417 SPOT 2D-000KG9: pI=5.61; Mw=82279 MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references ECO2DBASE G070.0; 6TH EDITION. ECO2DBASE G074.0; 6TH EDITION. UniProtKB/Swiss-Prot P09373; PFLB_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name PFLB_ECOLI Primary accession number P09373 Secondary accession number(s) P76826 Q47478 Sequence was last modified on  January 23, 2007 (version 2) Annotations were last modified on  October 19, 2011 (version 127) Name and origin of the protein Description RecName: Full=Formate acetyltransferase 1; EC=2.3.1.54; AltName: Full=Pyruvate formate-lyase 1; Gene name Name=pflB Synonyms=pfl OrderedLocusNames=b0903, JW0886 Encoded on Name=pflB; Synonyms=pfl; OrderedLocusNames=b0903, JW0886 Keywords 3D-structure; Acetylation; Acyltransferase; Carbohydrate metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Glucose metabolism; Organic radical; Reference proteome; Transferase. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL X08035; CAA30828.1; -; Genomic_DNA EMBL U00096; AAC73989.1; -; Genomic_DNA EMBL AP009048; BAA35638.1; -; Genomic_DNA EMBL M26413; AAA20391.1; -; Genomic_DNA PIR S01788; S01788; . RefSeq NP_415423.1; NC_000913.2; . PDB 1CM5; X-ray; 2.30 A; A/B=2-760 PDB 1H16; X-ray; 1.53 A; A=2-759 PDB 1H17; X-ray; 1.75 A; A=2-759 PDB 1H18; X-ray; 2.30 A; A/B=2-759 PDB 1MZO; X-ray; 2.70 A; A/B=2-759 PDB 1QHM; X-ray; 2.80 A; A/B=2-625 PDB 2PFL; X-ray; 2.90 A; A/B=2-760 PDB 3PFL; X-ray; 2.60 A; A/B=2-760 PDBsum 1CM5; -; . PDBsum 1H16; -; . PDBsum 1H17; -; . PDBsum 1H18; -; . PDBsum 1MZO; -; . PDBsum 1QHM; -; . PDBsum 2PFL; -; . PDBsum 3PFL; -; . ProteinModelPortal P09373; -; . SMR P09373; 2-760; . DIP DIP-10467N; -; . IntAct P09373; 41; . MINT MINT-1283506; -; . SWISS-2DPAGE P09373; -; . ECO2DBASE G070.0; 6TH EDITION; . ECO2DBASE G074.0; 6TH EDITION; . EnsemblBacteria EBESCT00000000292; EBESCP00000000292; EBESCG00000000242; . EnsemblBacteria EBESCT00000000293; EBESCP00000000293; EBESCG00000000242; . EnsemblBacteria EBESCT00000000294; EBESCP00000000294; EBESCG00000000242; . EnsemblBacteria EBESCT00000000295; EBESCP00000000295; EBESCG00000000242; . EnsemblBacteria EBESCT00000000296; EBESCP00000000296; EBESCG00000000242; . EnsemblBacteria EBESCT00000000297; EBESCP00000000297; EBESCG00000000242; . EnsemblBacteria EBESCT00000000298; EBESCP00000000298; EBESCG00000000242; . EnsemblBacteria EBESCT00000000299; EBESCP00000000299; EBESCG00000000242; . EnsemblBacteria EBESCT00000015891; EBESCP00000015182; EBESCG00000014951; . GeneID 945514; -; . GenomeReviews AP009048_GR; JW0886; . GenomeReviews U00096_GR; b0903; . KEGG ecj:JW0886; -; . KEGG eco:b0903; -; . EchoBASE EB0695; -; . EcoGene EG10701; pflB; . eggNOG COG1882; -; . GeneTree EBGT00050000009047; -; . HOGENOM HBG285825; -; . OMA FLRTPDY; -; . ProtClustDB CLSK869953; -; . BioCyc EcoCyc:PYRUVFORMLY-MONOMER; -; . BioCyc MetaCyc:PYRUVFORMLY-MONOMER; -; . BRENDA 2.3.1.54; 2026; . Genevestigator P09373; -; . GO GO:0005737; C:cytoplasm; IDA:UniProtKB; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0008861; F:formate C-acetyltransferase activity; IEA:EC; . GO GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW; . InterPro IPR005949; Form_AcTrfase; . InterPro IPR001150; Form_AcTrfase_GR; . InterPro IPR019777; Form_AcTrfase_GR_CS; . InterPro IPR004184; Pyr_form_lyase; . Pfam PF01228; Gly_radical; 1; . Pfam PF02901; PFL; 1; . PIRSF PIRSF000379; For_Ac_trans_1; 1; . TIGRFAMs TIGR01255; Pyr_form_ly_1; 1; . PROSITE PS00850; GLY_RADICAL_1; 1; . PROSITE PS51149; GLY_RADICAL_2; 1; . SWISS-2DPAGE (search AC)