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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: PNP_ECOLI |
SWISS-2DPAGE: PNP_ECOLI
PNP_ECOLI:
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ID PNP_ECOLI; STANDARD; 2DG.
AC P05055;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 15.
DE RecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8;
DE AltName: Full=Polynucleotide phosphorylase; Short=PNPase;.
GN Name=pnp; OrderedLocusNames=b3164, JW5851;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
RA Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL Microbiol. Rev. 47(1):231-284(1983).
RN [3]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=87194592; PubMed=3553157; [NCBI, Expasy, EBI, Israel, Japan]
RA Jones P.G., Vanbogelen R.A., Neidhardt F.C.;
RT ''''''Induction of proteins in response to low temperature in Escherichia coli'';'';'';
RL J. Bacteriol. 169(1):2092-2095(1987).
RN [5]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [6]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMOTRIMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KFR=5.10/83954;
2D -!- EXPRESSION: INCREASED DURING THE ADJUSTMENT OF E. COLI TO GROWTH AT
2D 10°C [4].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT
2D [2] AND VANBOGELEN [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014RF=5.13/87327;
2D -!- PEPTIDE MASSES: SPOT 2D-0014RF: 989.492; 991.478; 1488.772;
2D 1514.823; 1677.929; 2010.032; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [5].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WD2=5.16/80468;
2D -!- PI/MW: SPOT 2D-001WDK=5.15/78105;
2D -!- PEPTIDE MASSES: SPOT 2D-001WD2: 935.5; 991.45; 1116.64; 1206.65;
2D 1260.69; 1309.72; 1622.84; 1971.03; 2010.06; 2022.02; 2081.99; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WDK: (K)AKPGQDFFPLTVNYQER(T),50-66;
2D (R)EGRPSEGETLIAR(L),81-93; (R)YAQVDVIK(S),267-274;
2D (K)SETIATLLAEDETLDENELGEILHAIEK(N),275-302;
2D (R)GETQALVTATLGTAR(D),346-360; (R)IVDFGAFVAIGGGK(E),632-645.
2D -!- EXPRESSION: decrease after benzoic acid treatment [6].
2D -!- MAPPING: SPOT 2D-001WD2: Peptide mass fingerprinting [6]; SPOT
2D 2D-001WDK: Tandem mass spectrometry [6].
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DR ECO2DBASE; C078.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P05055; PNP_ECOLI.
//
The following 2-D maps are available for this protein:
|
Escherichia coli
 |
Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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