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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PPIA_HUMAN




SWISS-2DPAGE: PPIA_HUMAN



PPIA_HUMAN:

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ID   PPIA_HUMAN; STANDARD; 2DG.
AC   P62937; P05092;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Peptidyl-prolyl cis-trans isomerase A; Short=PPIase A;
DE   EC=5.2.1.8; AltName: Full=Cyclophilin A; AltName: Full=Cyclosporin
DE   A-binding protein; AltName: Full=Rotamase A;.
GN   Name=PPIA; Synonyms=CYPA;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, ELC_HUMAN, HL60_HUMAN, LIVER_HUMAN, LYMPHOCYTE_HUMAN,
MT   LYMPHOMA_HUMAN, U937_HUMAN.
IM   DLD1_HUMAN, ELC_HUMAN, HL60_HUMAN, LIVER_HUMAN, LYMPHOCYTE_HUMAN,
IM   LYMPHOMA_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [5]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [6]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC   -!- SUBUNIT: SINGLE CHAIN 
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001F3S=7.06/14905;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001F3S: 1055.51; 1154.55; 1310.53; 1946.03;
2D         3306.74; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000ERZ=6.96/14543;
2D   -!-   PI/MW: SPOT 2D-000ES0=7.22/14500;
2D   -!-   PI/MW: SPOT 2D-000ES6=6.79/14357;
2D   -!-   PI/MW: SPOT 2D-000ESD=8.11/13730;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001007=6.70/13227;
2D   -!-   PI/MW: SPOT 2D-00100F=6.44/13193;
2D   -!-   PI/MW: SPOT 2D-00100I=7.08/13159;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3][4].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001KO=6.69/14545;
2D   -!-   PI/MW: SPOT 2D-0001KQ=7.07/14514;
2D   -!-   PI/MW: SPOT 2D-0001KW=7.60/14514;
2D   -!-   PI/MW: SPOT 2D-0001KX=8.03/14514;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YFD=7.82/17900;
2D   -!-   PI/MW: SPOT 2D-001YFH=7.31/17780;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001YFD: 1055.5352; 1154.5587; 1247.6071;
2D         1310.5507; 1379.7348; 1423.735; 1537.7165; 1614.7363; 1831.906;
2D         1946.0415; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001YFH: 1055.5352; 1154.5587; 1247.6071;
2D         1310.5507; 1379.7348; 1423.735; 1537.7165; 1614.7363; 1831.906;
2D         1946.0415; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [6].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00089K=7.11/14927;
2D   -!-   PI/MW: SPOT 2D-00089L=7.54/14849;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D5X=7.15/13972;
2D   -!-   PI/MW: SPOT 2D-000D5Z=7.44/13808;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P62937; P62937.
DR   DOSAC-COBS-2DPAGE; P62937; PPIA_HUMAN.
DR   OGP-WWW; P05092.
DR   PMMA-2DPAGE; P05092; PPIA_HUMAN.
DR   REPRODUCTION-2DPAGE; P62937; P62937.
DR   REPRODUCTION-2DPAGE; IPI00419585; IPI00419585.
DR   Siena-2DPAGE; P05092; PPIA_HUMAN.
DR   UCD-2DPAGE; P62937; PPIA_HUMAN.
DR   UniProtKB/Swiss-Prot; P62937; PPIA_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Liver
LIVER_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Lymphoma
LYMPHOMA_HUMAN
Macrophage Like Cell Line
U937_HUMAN
  


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server