Sample Preparation and Post-separation Analysis

PSB4_HUMAN:

ID   PSB4_HUMAN; STANDARD; 2DG.
AC   P28070;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=Proteasome subunit beta type-4; Short=Proteasome beta
DE   chain; EC=3.4.25.1; AltName: Full=26 kDa prosomal protein;
DE   Short=HsBPROS26; Short=PROS-26; AltName: Full=Macropain beta chain;
DE   AltName: Full=Multicatalytic endopeptidase complex beta chain; AltName:
DE   Full=Proteasome chain 3; Short=HsN3; Flags: Precursor;.
GN   Name=PSMB4; Synonyms=PROS26;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
IM   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=94147969; PubMed=8313870; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot
RA   J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.;
RT   ''''''Human liver protein map: update 1993'';'';'';
RL   Electrophoresis 14(1):1216-1222(1993).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [4]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC   -!- SUBUNIT: THE PROTEASOME IS COMPOSED OF AT LEAST 12 NON IDENTICAL
CC         SUBUNITS WHICH FORM A HIGHLY ORDERED RING-SHAPED STRUCTURE
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001DP=5.50/24353;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YDK=5.47/24400;
2D   -!-   MAPPING: GEL MATCHING WITH THE LYMPHOMA MASTER GEL [4].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000851=5.50/24300;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0006JL=5.50/24300;
2D   -!-   MAPPING: MICROSEQUENCING [2].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D4M=5.50/24255;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P28070; P28070.
DR   DOSAC-COBS-2DPAGE; P28070; PSB4_HUMAN.
DR   OGP-WWW; P28070.
DR   REPRODUCTION-2DPAGE; IPI00555956; IPI00555956.
DR   UniProtKB/Swiss-Prot; P28070; PSB4_HUMAN.
//

The following 2-D maps are available for this protein:

	Liver	Lymphocytes	Lymphoma
	Red blood cells	Macrophage Like Cell Line

SWISS-2DPAGE (search AC)