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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PTGA_ECOLI




SWISS-2DPAGE:  PTGA_ECOLI


PTGA_ECOLI


General information about the entry
View entry in simple text format
Entry namePTGA_ECOLI
Primary accession numberP69783
Secondary accession number(s) P08837
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 4)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Glucose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIIA-Glc; AltName: Full=EIII-Glc; AltName: Full=PTS system glucose-specific EIIA component;.
Gene nameName=crr
Synonyms=gsr, iex, tgs, treD
OrderedLocusNames=b2417, JW2410
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.56; Mw=20073  [identification data]
pI=4.68; Mw=18975

MAPPING (identification):
SPOT 2D-000LLY: AMINO ACID COMPOSITION AND SEQUENCE TAG (GLFD) [3];
SPOT 2D-000LMU: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=4.95; Mw=14800  [identification data]
pI=4.86; Mw=14849  [identification data]
pI=4.95; Mw=13907  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4];
SPOT 2D-001HN8: MICROSEQUENCING (GLFD) [4].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=4.94; Mw=18465

MAPPING (identification):
MICROSEQUENCING (GLFD) [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP69783; PTGA_ECOLI.
ECO2DBASEB018.7; 6TH EDITION.
UniProtKB/Swiss-ProtP69783; PTGA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePTGA_ECOLI
Primary accession numberP69783
Secondary accession number(s) P08837 Q47703
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 67)
Name and origin of the protein
DescriptionRecName: Full=Glucose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIIA-Glc; AltName: Full=EIII-Glc; AltName: Full=PTS system glucose-specific EIIA component;
Gene nameName=crr
Synonyms=gsr, iex, tgs, treD
OrderedLocusNames=b2417, JW2410
Encoded onName=crr; Synonyms=gsr, iex, tgs, treD; OrderedLocusNames=b2417, JW2410
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ02796; AAA24442.1; -; Genomic_DNA
EMBLM21994; AAA24386.1; -; Genomic_DNA
EMBLM93578; AAA23602.1; -; Genomic_DNA
EMBLM93579; AAA23605.1; -; Genomic_DNA
EMBLM93580; AAA23603.1; -; Genomic_DNA
EMBLM93581; AAA23606.1; -; Genomic_DNA
EMBLM93582; AAA23604.1; -; Genomic_DNA
EMBLM93584; AAA23610.1; -; Genomic_DNA
EMBLM93587; AAA23612.1; -; Genomic_DNA
EMBLM93594; AAA23607.1; -; Genomic_DNA
EMBLM93595; AAA23608.1; -; Genomic_DNA
EMBLM93596; AAA23611.1; -; Genomic_DNA
EMBLM93597; AAA23609.1; -; Genomic_DNA
EMBLM93598; AAA23613.1; -; Genomic_DNA
EMBLU53700; AAC44167.1; -; Genomic_DNA
EMBLU00096; AAC75470.1; -; Genomic_DNA
EMBLAP009048; BAA16291.1; -; Genomic_DNA
PIRC29785; WQECP3; .
RefSeqNP_416912.1; NC_000913.2; .
PDB1F3G; X-ray; 2.10 A; A=9-169
PDB1F3Z; X-ray; 1.98 A; A=9-169
PDB1GGR; NMR; -; A=2-168
PDB1GLA; X-ray; 2.60 A; F=2-169
PDB1GLB; X-ray; 2.60 A; F=2-169
PDB1GLC; X-ray; 2.65 A; F=2-169
PDB1GLD; X-ray; 2.93 A; F=2-169
PDB1GLE; X-ray; 2.94 A; F=2-169
PDB1O2F; NMR; -; A=2-168
PDB2F3G; X-ray; 2.13 A; A/B=2-169
PDBsum1F3G; -; .
PDBsum1F3Z; -; .
PDBsum1GGR; -; .
PDBsum1GLA; -; .
PDBsum1GLB; -; .
PDBsum1GLC; -; .
PDBsum1GLD; -; .
PDBsum1GLE; -; .
PDBsum1O2F; -; .
PDBsum2F3G; -; .
ProteinModelPortalP69783; -; .
SMRP69783; 20-169; .
DIPDIP-31863N; -; .
IntActP69783; 4; .
TCDB4.A.1.1.1; PTS glucose-glucoside (Glc) family; .
PhosSiteP69783; -; .
SWISS-2DPAGEP69783; -; .
2DBase-EcoliP69783; -; .
ECO2DBASEB018.7; 6TH EDITION; .
PRIDEP69783; -; .
EnsemblBacteriaEBESCT00000001185; EBESCP00000001185; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000001186; EBESCP00000001186; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000001187; EBESCP00000001187; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000001188; EBESCP00000001188; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000001189; EBESCP00000001189; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000001190; EBESCP00000001190; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000001191; EBESCP00000001191; EBESCG00000000980; .
EnsemblBacteriaEBESCT00000018235; EBESCP00000017526; EBESCG00000017290; .
GeneID946880; -; .
GenomeReviewsAP009048_GR; JW2410; .
GenomeReviewsU00096_GR; b2417; .
KEGGecj:JW2410; -; .
KEGGeco:b2417; -; .
EchoBASEEB0163; -; .
EcoGeneEG10165; crr; .
eggNOGCOG2190; -; .
GeneTreeEBGT00050000009158; -; .
HOGENOMHBG517865; -; .
OMARRLISGQ; -; .
ProtClustDBPRK09439; -; .
BioCycEcoCyc:CRR-MONOMER; -; .
BioCycMetaCyc:CRR-MONOMER; -; .
GenevestigatorP69783; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0003883; F:CTP synthase activity; IDA:EcoCyc; .
GOGO:0016301; F:kinase activity; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro; .
GOGO:0006241; P:CTP biosynthetic process; IDA:EcoCyc; .
GOGO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW; .
InterProIPR011055; Dup_hybrid_motif; .
InterProIPR001127; PTS_EIIA_1_perm; .
PfamPF00358; PTS_EIIA_1; 1; .
SUPFAMSSF51261; Dup_hybrid_motif; 1; .
TIGRFAMsTIGR00830; PTBA; 1; .
PROSITEPS51093; PTS_EIIA_TYPE_1; 1; .
PROSITEPS00371; PTS_EIIA_TYPE_1_HIS; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server