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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: PTGA_ECOLI |
SWISS-2DPAGE: PTGA_ECOLI
PTGA_ECOLI:
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ID PTGA_ECOLI; STANDARD; 2DG.
AC P69783; P08837;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Glucose-specific phosphotransferase enzyme IIA component;
DE EC=2.7.1.-; AltName: Full=EIIA-Glc; AltName: Full=EIII-Glc; AltName:
DE Full=PTS system glucose-specific EIIA component;.
GN Name=crr; Synonyms=gsr, iex, tgs, treD; OrderedLocusNames=b2417, JW2410;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000LLY=4.56/20073;
2D -!- PI/MW: SPOT 2D-000LMU=4.68/18975;
2D -!- MAPPING: SPOT 2D-000LLY: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D (GLFD) [3]; SPOT 2D-000LMU: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON
2D 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HN8=4.95/14800;
2D -!- PI/MW: SPOT 2D-001HN9=4.86/14849;
2D -!- PI/MW: SPOT 2D-001HNL=4.95/13907;
2D -!- PEPTIDE MASSES: SPOT 2D-001HN8: 958.508; 1087.575; 1103.539;
2D 1382.771; 1414.777; 1646.816; 1662.788; 1816.889; 2044.026; 2130.075;
2D 3083.376; 3380.466; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HN9: 891.435; 958.512; 1087.556;
2D 1103.603; 1382.806; 1414.806; 1646.84; 1662.849; 1816.946; 2044.122;
2D 2130.177; 2146.133; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HNL: 1382.78; 1414.81; 1646.908;
2D 1662.84; 1816.961; 3083.468; 3380.696; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4]; SPOT 2D-001HN8:
2D MICROSEQUENCING (GLFD) [4].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015DT=4.94/18465;
2D -!- MAPPING: MICROSEQUENCING (GLFD) [4].
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DR 2DBase-Ecoli; P69783; PTGA_ECOLI.
DR ECO2DBASE; B018.7; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P69783; PTGA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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SWISS-2DPAGE (search AC)
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