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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PYRG_ECOLI




SWISS-2DPAGE:  PYRG_ECOLI


PYRG_ECOLI


General information about the entry
View entry in simple text format
Entry namePYRG_ECOLI
Primary accession numberP0A7E5
Secondary accession number(s) P08398
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 6)
Name and origin of the protein
DescriptionRecName: Full=CTP synthase; EC=6.3.4.2; AltName: Full=CTP synthetase; AltName: Full=UTP--ammonia ligase;.
Gene nameName=pyrG
OrderedLocusNames=b2780, JW2751
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: EXISTS AS A DIMER OF IDENTICAL CHAINS THAT AGGREGATES TO A TETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.61; Mw=58475  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEG060.3; 6TH EDITION.
UniProtKB/Swiss-ProtP0A7E5; PYRG_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePYRG_ECOLI
Primary accession numberP0A7E5
Secondary accession number(s) P08398 Q2MA52
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 66)
Name and origin of the protein
DescriptionRecName: Full=CTP synthase; EC=6.3.4.2; AltName: Full=CTP synthetase; AltName: Full=UTP--ammonia ligase;
Gene nameName=pyrG
OrderedLocusNames=b2780, JW2751
Encoded onName=pyrG; OrderedLocusNames=b2780, JW2751
Keywords3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Glutamine amidotransferase; Ligase; Nucleotide-binding; Pyrimidine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM12843; AAA24485.1; -; mRNA
EMBLU29580; AAA69290.1; -; Genomic_DNA
EMBLU00096; AAC75822.1; -; Genomic_DNA
EMBLAP009048; BAE76854.1; -; Genomic_DNA
PIRH65059; SYECTP; .
RefSeqNP_417260.1; NC_000913.2; .
PDB1S1M; X-ray; 2.30 A; A/B=1-545
PDB2AD5; X-ray; 2.80 A; A/B=1-545
PDBsum1S1M; -; .
PDBsum2AD5; -; .
ProteinModelPortalP0A7E5; -; .
SMRP0A7E5; 1-545; .
IntActP0A7E5; 8; .
MEROPSC26.A18; -; .
SWISS-2DPAGEP0A7E5; -; .
ECO2DBASEG060.3; 6TH EDITION; .
PRIDEP0A7E5; -; .
EnsemblBacteriaEBESCT00000002549; EBESCP00000002549; EBESCG00000002082; .
EnsemblBacteriaEBESCT00000002550; EBESCP00000002550; EBESCG00000002082; .
EnsemblBacteriaEBESCT00000016120; EBESCP00000015411; EBESCG00000015180; .
GeneID946116; -; .
GenomeReviewsAP009048_GR; JW2751; .
GenomeReviewsU00096_GR; b2780; .
KEGGecj:JW2751; -; .
KEGGeco:b2780; -; .
EchoBASEEB0803; -; .
EcoGeneEG10810; pyrG; .
eggNOGCOG0504; -; .
GeneTreeEBGT00050000011907; -; .
HOGENOMHBG597806; -; .
OMARVTMQKL; -; .
ProtClustDBPRK05380; -; .
BioCycEcoCyc:CTPSYN-MONOMER; -; .
BioCycMetaCyc:CTPSYN-MONOMER; -; .
BRENDA6.3.4.2; 2026; .
GenevestigatorP0A7E5; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0003883; F:CTP synthase activity; IDA:EcoCyc; .
GOGO:0006241; P:CTP biosynthetic process; IDA:EcoCyc; .
GOGO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW; .
HAMAPMF_01227; PyrG; 1; -
InterProIPR004468; CTP_synthase; .
InterProIPR017456; CTP_synthase_N; .
InterProIPR017926; GATASE_1; .
PANTHERPTHR11550; PyrG_synth; 1; .
PfamPF06418; CTP_synth_N; 1; .
PfamPF00117; GATase; 1; .
TIGRFAMsTIGR00337; PyrG; 1; .
PROSITEPS51273; GATASE_TYPE_1; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server