Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

PYRI_ECOLI:

ID   PYRI_ECOLI; STANDARD; 2DG.
AC   P0A7F3; P00478;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Aspartate carbamoyltransferase regulatory chain;.
GN   Name=pyrI; OrderedLocusNames=b4244, JW4203;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI6-11.
IM   ECOLI, ECOLI6-11.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
RA   Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT   ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL   Microbiol. Rev. 47(1):231-284(1983).
RN   [3]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
CC   -!- SUBUNIT: CONTAINS SIX CATALYTIC AND SIX REGULATORY CHAINS 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LOK=6.84/16989;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D         IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].
2D   -!- MASTER: ECOLI6-11;
2D   -!-   PI/MW: SPOT 2D-001TTN=7.01/17642;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001TTN: 941.724; 956.831; 1458.173;
2D         1474.158; 1496.219; 1652.36; 2301.785; 2836.211; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
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DR   ECO2DBASE; I017.5; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A7F3; PYRI_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli(6-11)

SWISS-2DPAGE (search AC)