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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: Q43127




SWISS-2DPAGE:  Q43127


Q43127


General information about the entry
View entry in simple text format
Entry nameGLNA2_ARATH
Primary accession numberQ43127
integrated into SWISS-2DPAGE on December 1, 2000 (release 13)
2D Annotations were last modified onNovember 9, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=Glutamine synthetase, chloroplastic/mitochondrial; EC=6.3.1.2; AltName: Full=GS2; AltName: Full=Glutamate--ammonia ligase; Flags: Precursor;.
Gene nameName=GLN2
Synonyms=GSLI
OrderedLocusNames=At5g35630
ORFNames=MJE4.9
Annotated speciesArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
TaxonomyEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core; eudicotyledons; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
References
[1]   MAPPING ON GEL
Sarazin B., Tonella L., Marques K., Paesano S., Chane-Favre L., Sanchez J.-C., Hochstrasser D.F., Thiellement H.
Submitted (OCT-2000) to SWISS-2DPAGE
Comments
  • MISCELLANEOUS: POSSIBLE SIGNAL/TRANSIT 1-40
2D PAGE maps for identified proteins
How to interpret a protein

ARABIDOPSIS {Arabidopsis thaliana}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
ARABIDOPSIS
  map experimental info
  protein estimated location
 
ARABIDOPSIS

MAP LOCATIONS:
pI=5.26; Mw=44124  [identification data]

MAPPING (identification):
MASS SPECTROMETRY [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtQ43127; GLNA2_ARATH.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGLNA2_ARATH
Primary accession numberQ43127
Sequence was last modified on November 1, 1996 (version 1)
Annotations were last modified on October 19, 2011 (version 95)
Name and origin of the protein
DescriptionRecName: Full=Glutamine synthetase, chloroplastic/mitochondrial; EC=6.3.1.2; AltName: Full=GS2; AltName: Full=Glutamate--ammonia ligase; Flags: Precursor;
Gene nameName=GLN2
Synonyms=GSLI
OrderedLocusNames=At5g35630
ORFNames=MJE4.9
Encoded onName=GLN2; Synonyms=GSLI; OrderedLocusNames=At5g35630; ORFNames=MJE4.9
KeywordsATP-binding; Chloroplast; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Plastid; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLS69727; AAB20558.1; -; mRNA
EMBLAB015045; BAA88761.1; -; Genomic_DNA
EMBLAB013393; BAB09304.1; -; Genomic_DNA
EMBLCP002688; AED93993.1; -; Genomic_DNA
EMBLCP002688; AED93994.1; -; Genomic_DNA
EMBLCP002688; AED93995.1; -; Genomic_DNA
EMBLAF428319; AAL16249.1; -; mRNA
EMBLAF428461; AAL16230.1; -; mRNA
EMBLAY081252; AAL91141.1; -; mRNA
EMBLAY091114; AAM14064.1; -; mRNA
EMBLAY122977; AAM67510.1; -; mRNA
EMBLAY088222; AAM65763.1; -; mRNA
IPIIPI00534852; -; .
PIRS18600; S18600; .
RefSeqNP_001031969.1; NM_001036892.1; .
RefSeqNP_001078639.1; NM_001085170.1; .
RefSeqNP_198413.1; NM_122954.3; .
UniGeneAt.24218; -; .
ProteinModelPortalQ43127; -; .
SMRQ43127; 65-413; .
DIPDIP-32735N; -; .
IntActQ43127; 1; .
STRINGQ43127; -; .
SWISS-2DPAGEQ43127; -; .
PRIDEQ43127; -; .
EnsemblPlantsAT5G35630.1; AT5G35630.1; AT5G35630; .
EnsemblPlantsAT5G35630.2; AT5G35630.2; AT5G35630; .
EnsemblPlantsAT5G35630.3; AT5G35630.3; AT5G35630; .
GeneID833535; -; .
GenomeReviewsBA000015_GR; AT5G35630; .
KEGGath:AT5G35630; -; .
NMPDRfig|3702.1.peg.25264; -; .
TAIRAt5g35630; -; .
eggNOGKOG0683; -; .
HOGENOMHBG299709; -; .
InParanoidQ43127; -; .
OMATLDPKPI; -; .
PhylomeDBQ43127; -; .
ProtClustDBPLN03036; -; .
ArrayExpressQ43127; -; .
GenevestigatorQ43127; -; .
GermOnlineAT5G35630; Arabidopsis thaliana; .
GOGO:0048046; C:apoplast; IDA:TAIR; .
GOGO:0009941; C:chloroplast envelope; IDA:TAIR; .
GOGO:0009570; C:chloroplast stroma; IDA:TAIR; .
GOGO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR; .
GOGO:0022626; C:cytosolic ribosome; IDA:TAIR; .
GOGO:0005739; C:mitochondrion; IDA:TAIR; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004356; F:glutamate-ammonia ligase activity; IDA:TAIR; .
GOGO:0007568; P:aging; TAS:TAIR; .
GOGO:0019676; P:ammonia assimilation cycle; TAS:TAIR; .
GOGO:0006542; P:glutamine biosynthetic process; IEA:InterPro; .
GOGO:0046686; P:response to cadmium ion; IEP:TAIR; .
GOGO:0009651; P:response to salt stress; IEP:TAIR; .
InterProIPR008147; Gln_synt_beta; .
InterProIPR014746; Gln_synth/guanido_kin_cat_dom; .
InterProIPR008146; Gln_synth_cat_dom; .
Gene3DG3DSA:3.30.590.10; ATP-gua_Ptrans; 1; .
PfamPF00120; Gln-synt_C; 1; .
PfamPF03951; Gln-synt_N; 1; .
SUPFAMSSF54368; Gln_synt_beta; 1; .
PROSITEPS00180; GLNA_1; 1; .
PROSITEPS00181; GLNA_ATP; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server