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Sample Preparation and Post-separation Analysis

Q7A338:

ID   DRP35_STAAN; STANDARD; 2DG.
AC   Q7A338; Q99QV3;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   30-DEC-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 7.
DE   RecName: Full=Lactonase drp35; EC=3.1.1.-;.
GN   Name=drp35; OrderedLocusNames=SA2480;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XZX=4.88/33491;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XZX: (M)SQQDLPTLFYSGK(S),2-14;
2D         (K)KGLQLEGLNFDR(Q),41-52; (K)GLQLEGLNFDR(Q),42-52;
2D         (K)GGFYFTDFR(G),144-152; (R)GYSTNPLGGVYYVSPDFR(T),153-170;
2D         (R)GYPIGQILIPGR(D),261-272; (K)GHQSFQFQ(-),316-323.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; Q7A338; DRP35_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)