Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

Q9BVP2:

ID   GNL3_HUMAN; PRELIMINARY; 2DG.
AC   Q9BVP2;
DT   09-NOV-2001, integrated into SWISS-2DPAGE (release 15).
DT   30-DEC-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 7.
DE   RecName: Full=Guanine nucleotide-binding protein-like 3; AltName:
DE   Full=E2-induced gene 3 protein; AltName: Full=Novel nucleolar protein 47;
DE   Short=NNP47; AltName: Full=Nucleolar GTP-binding protein 3; AltName: Full=Nucleostemin;.
GN   Name=GNL3; Synonyms=E2IG3, NS;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8A=66828;
1D   -!-   MW: BAND 1D-001V8B=65137;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8A: (K)VIEASDVVLEVLDAR(D),137-151; (K)LLGGFQETCSK(A),243-253.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8B: (K)KLVLILNK(S),172-179;
1D         (K)LLGGFQETCSK(A),243-253; (R)VGVIGFPNVGK(S),257-267;
1D         (K)SGFNLEELEK(N),425-434; (K)DIHEELPK(R),468-475; (K)IIEEDDAYDFSTDYV(-),535-549.
1D   -!-   MAPPING: Tandem mass spectrometry [1]; BAND 1D-001V8B: PEPTIDE MASS
1D         FINGERPRINTING [1].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VHW=6.47/64772;
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; Q9BVP2; GNL3_HUMAN.
//

The following 2-D maps are available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells	2D-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)