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Sample Preparation and Post-separation Analysis

Q9NQ55:

ID   SSF1_HUMAN; STANDARD; 2DG.
AC   Q9NQ55;
DT   15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT   15-MAY-2003, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 6.
DE   RecName: Full=Suppressor of SWI4 1 homolog; Short=Ssf-1; AltName:
DE   Full=Brix domain-containing protein 3; AltName: Full=Peter Pan homolog;.
GN   Name=PPAN; Synonyms=BXDC3, SSF1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8D=61882;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8D: (R)NLEAYAANPHSFVFTR(G),21-36;
1D         (K)DCVAVAGPLGVTHFLILSK(T),72-90; (R)LQDISELLATGAGLSESEAEPDGDHNITELPQAVAGR(G),224-260.
1D   -!-   MAPPING: Tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; Q9NQ55; SSF1_HUMAN.
//

The following 2-D map is available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)