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Sample Preparation and Post-separation Analysis

Q9NZM5:

ID   GSCR2_HUMAN; STANDARD; 2DG.
AC   Q9NZM5;
DT   09-NOV-2001, integrated into SWISS-2DPAGE (release 15).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 6.
DE   RecName: Full=Glioma tumor suppressor candidate region gene 2 protein;
DE   AltName: Full=p60;.
GN   Name=GLTSCR2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8A=66828;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8A: (R)LAQEPLGLEVDQFLEDVR(L),51-68;
1D         (R)TSGGLLSEAPNEK(L),73-85; (K)YQAPDIDVQLSSELTDSLR(T),411-429.
1D   -!-   MAPPING: Tandem mass spectrometry [1].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VHW=6.47/64772;
2D   -!-   PI/MW: SPOT 2D-001VI0=6.34/64287;
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
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DR   UniProtKB/Swiss-Prot; Q9NZM5; GSCR2_HUMAN.
//

The following 2-D maps are available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells	2D-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)