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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: RMLA1_ECOLI SWISS-2DPAGE:  RMLA1_ECOLI RMLA1_ECOLI General information about the entry View entry in simple text format Entry name RMLA1_ECOLI Primary accession number P37744 integrated into SWISS-2DPAGE on  May 15, 2003 (release 16) 2D Annotations were last modified on December 30, 2004 (version 1) General Annotations were last modified on  May 19, 2011 (version 7) Name and origin of the protein Description RecName: Full=Glucose-1-phosphate thymidylyltransferase 1; Short=G1P-TT 1; EC=2.7.7.24; AltName: Full=dTDP-glucose pyrophosphorylase 1; AltName: Full=dTDP-glucose synthase 1;. Gene name Name=rmlA1 Synonyms=rfbA, rmlA OrderedLocusNames=b2039, JW2024 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) 2D PAGE maps for identified proteins How to interpret a protein ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WQU: pI=5.41; Mw=32544   [identification data] EXPRESSION: decrease after benzoic acid treatment [1]. MAPPING (identification): Tandem mass spectrometry [1]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references UniProtKB/Swiss-Prot P37744; RMLA1_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name RMLA1_ECOLI Primary accession number P37744 Secondary accession number(s) P78081 Sequence was last modified on  November 1, 1997 (version 2) Annotations were last modified on  October 19, 2011 (version 98) Name and origin of the protein Description RecName: Full=Glucose-1-phosphate thymidylyltransferase 1; Short=G1P-TT 1; EC=2.7.7.24; AltName: Full=dTDP-glucose pyrophosphorylase 1; AltName: Full=dTDP-glucose synthase 1; Gene name Name=rmlA1 Synonyms=rfbA, rmlA OrderedLocusNames=b2039, JW2024 Encoded on Name=rmlA1; Synonyms=rfbA, rmlA; OrderedLocusNames=b2039, JW2024 Keywords 3D-structure; Complete proteome; Lipopolysaccharide biosynthesis; Magnesium; Metal-binding; Nucleotidyltransferase; Reference proteome; Transferase. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL U09876; AAB88400.1; -; Genomic_DNA EMBL U00096; AAC75100.1; -; Genomic_DNA EMBL AP009048; BAA15881.1; -; Genomic_DNA EMBL U03041; AAC31629.1; -; Genomic_DNA PIR F64969; F64969; . RefSeq NP_416543.1; NC_000913.2; . PDB 1H5R; X-ray; 1.90 A; A/B/C/D=1-293 PDB 1H5S; X-ray; 2.30 A; A/B/C/D=1-293 PDB 1H5T; X-ray; 1.90 A; A/B/C/D=1-293 PDBsum 1H5R; -; . PDBsum 1H5S; -; . PDBsum 1H5T; -; . ProteinModelPortal P37744; -; . SMR P37744; 2-291; . IntAct P37744; 9; . SWISS-2DPAGE P37744; -; . EnsemblBacteria EBESCT00000001700; EBESCP00000001700; EBESCG00000001402; . EnsemblBacteria EBESCT00000018315; EBESCP00000017606; EBESCG00000017369; . GeneID 945154; -; . GenomeReviews AP009048_GR; JW2024; . GenomeReviews U00096_GR; b2039; . KEGG ecj:JW2024; -; . KEGG eco:b2039; -; . EchoBASE EB1921; -; . EcoGene EG11978; rmlA1; . eggNOG COG1209; -; . GeneTree EBGT00050000009640; -; . HOGENOM HBG688195; -; . OMA YRAGWID; -; . ProtClustDB PRK15480; -; . BioCyc EcoCyc:DTDPGLUCOSEPP-MONOMER; -; . BioCyc MetaCyc:DTDPGLUCOSEPP-MONOMER; -; . Genevestigator P37744; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0008879; F:glucose-1-phosphate thymidylyltransferase activity; IEA:EC; . GO GO:0046872; F:metal ion binding; IEA:UniProtKB-KW; . GO GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro; . GO GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW; . InterPro IPR005907; G1P_thy_trans_s; . InterPro IPR005835; NTP_transferase; . PANTHER PTHR22572:SF13; PTHR22572:SF13; 1; . Pfam PF00483; NTP_transferase; 1; . TIGRFAMs TIGR01207; RmlA; 1; . SWISS-2DPAGE (search AC)