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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: RPOA_ECOLI




SWISS-2DPAGE: RPOA_ECOLI



RPOA_ECOLI:

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ID   RPOA_ECOLI; STANDARD; 2DG.
AC   P0A7Z4; P00574;
DT   01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 15.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP
DE   subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha;
DE   AltName: Full=Transcriptase subunit alpha;.
GN   Name=rpoA; Synonyms=pez, phs, sez; OrderedLocusNames=b3295, JW3257;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-001527=5.03/39816;
2D   -!-   PI/MW: SPOT 2D-00152B=5.06/39655;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001527: 956.563; 1006.531; 1142.619;
2D         1408.76; 1421.724; 1437.761; 1484.798; 1759.936; 2046.046; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-00152B: 956.478; 1006.469; 1142.53;
2D         1408.647; 1437.629; 1484.707; 1725.862; 1759.862; 2045.907; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WMR=5.03/39691;
2D   -!-   PI/MW: SPOT 2D-001WMU=5.07/39523;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WMR: 850.44; 956.48; 970.41; 1006.48;
2D         1063.47; 1142.51; 1298.56; 1331.62; 1392.56; 1421.6; 1426.6; 1437.58;
2D         1456.69; 1484.65; 1725.79; 1759.8; 2623.07; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WMU: 850.43; 956.48; 970.46; 1006.46;
2D         1063.46; 1142.51; 1298.57; 1331.63; 1392.59; 1421.6; 1437.57; 1456.7;
2D         1484.66; 1725.76; 1759.8; 1915.84; 2157.87; 2222.02; 2623.09; 2800.06; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WMU: (K)EGVQEDILEILLNLK(G),72-86;
2D         (R)IHSEEDERPIGR(L),159-170; (R)LLVDACYSPVER(I),171-182; (K)SLTEIKDVLASR(G),299-310.
2D   -!-   MAPPING: Peptide mass fingerprinting [2]; SPOT 2D-001WMU: TANDEM
2D         MASS SPECTROMETRY [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; B040.7; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A7Z4; RPOA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server