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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: RPOB_ECOLI




SWISS-2DPAGE:  RPOB_ECOLI


RPOB_ECOLI


General information about the entry
View entry in simple text format
Entry nameRPOB_ECOLI
Primary accession numberP0A8V2
Secondary accession number(s) P00575
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase subunit beta; Short=RNAP subunit beta; EC=2.7.7.6; AltName: Full=RNA polymerase subunit beta; AltName: Full=Transcriptase subunit beta;.
Gene nameName=rpoB
Synonyms=groN, nitB, rif, ron, stl, stv, tabD
OrderedLocusNames=b3987, JW3950
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
Comments
  • SUBUNIT: THE ENZYME CONSISTS OF THE SIGMA CHAIN AND THE CORE ENZYME WHICH IS COMPOSED OF 5 CHAINS
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.12; Mw=150615

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASED157.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0A8V2; RPOB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRPOB_ECOLI
Primary accession numberP0A8V2
Secondary accession number(s) P00575 P00576 P78242 Q2M8S3
Sequence was last modified on June 7, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 72)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase subunit beta; Short=RNAP subunit beta; EC=2.7.7.6; AltName: Full=RNA polymerase subunit beta; AltName: Full=Transcriptase subunit beta;
Gene nameName=rpoB
Synonyms=groN, nitB, rif, ron, stl, stv, tabD
OrderedLocusNames=b3987, JW3950
Encoded onName=rpoB; Synonyms=groN, nitB, rif, ron, stl, stv, tabD; OrderedLocusNames=b3987, JW3950
Keywords3D-structure; Acetylation; Complete proteome; DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00339; CAA23625.1; -; Genomic_DNA
EMBLV00340; CAA23627.1; -; Genomic_DNA
EMBLU76222; AAB18647.1; -; Genomic_DNA
EMBLU00006; AAC43085.1; -; Genomic_DNA
EMBLU00096; AAC76961.1; -; Genomic_DNA
EMBLAP009048; BAE77333.1; -; Genomic_DNA
EMBLV00341; CAA23629.1; -; Genomic_DNA
EMBLM38292; AAA24579.1; -; Genomic_DNA
EMBLM38293; AAA24581.1; -; Genomic_DNA
EMBLM38287; AAA24585.1; -; Genomic_DNA
EMBLM38304; AAA24580.1; -; Genomic_DNA
EMBLU77436; AAD09605.1; -; Genomic_DNA
EMBLM38303; AAA24583.1; -; Genomic_DNA
PIRF65205; RNECB; .
RefSeqNP_418414.1; NC_000913.2; .
PDB3IYD; EM; -; C=1-1342
PDB3LTI; X-ray; 1.60 A; A=152-443
PDB3LU0; EM; -; C=1-1342
PDBsum3IYD; -; .
PDBsum3LTI; -; .
PDBsum3LU0; -; .
ProteinModelPortalP0A8V2; -; .
SMRP0A8V2; 10-942; 1179-1342; .
DIPDIP-35777N; -; .
IntActP0A8V2; 97; .
MINTMINT-1222430; -; .
SWISS-2DPAGEP0A8V2; -; .
ECO2DBASED157.0; 6TH EDITION; .
EnsemblBacteriaEBESCT00000000939; EBESCP00000000939; EBESCG00000000776; .
EnsemblBacteriaEBESCT00000000940; EBESCP00000000940; EBESCG00000000776; .
EnsemblBacteriaEBESCT00000000941; EBESCP00000000941; EBESCG00000000776; .
EnsemblBacteriaEBESCT00000017696; EBESCP00000016987; EBESCG00000016752; .
GeneID948488; -; .
GenomeReviewsAP009048_GR; JW3950; .
GenomeReviewsU00096_GR; b3987; .
KEGGecj:JW3950; -; .
KEGGeco:b3987; -; .
EchoBASEEB0887; -; .
EcoGeneEG10894; rpoB; .
eggNOGCOG0085; -; .
GeneTreeEBGT00050000011394; -; .
HOGENOMHBG285547; -; .
OMAVDARDTK; -; .
ProtClustDBPRK00405; -; .
BioCycEcoCyc:RPOB-MONOMER; -; .
BioCycMetaCyc:RPOB-MONOMER; -; .
DrugBankDB01045; Rifampin; .
DrugBankDB01220; Rifaximin; .
GenevestigatorP0A8V2; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0003677; F:DNA binding; IEA:InterPro; .
GOGO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0032549; F:ribonucleoside binding; IEA:InterPro; .
HAMAPMF_01321; RNApol_bact_RpoB; 1; -
InterProIPR010243; DNA-dir_RNA_pol_bsu_bac-type; .
InterProIPR019462; DNA-dir_RNA_pol_bsu_external_1; .
InterProIPR015712; DNA-dir_RNA_pol_su2; .
InterProIPR007120; DNA-dir_RNA_pol_su2_6; .
InterProIPR007121; RNA_pol_bsu_CS; .
InterProIPR007644; RNA_pol_bsu_protrusion; .
InterProIPR007642; RNA_pol_Rpb2_2; .
InterProIPR007645; RNA_pol_Rpb2_3; .
InterProIPR007641; RNA_pol_Rpb2_7; .
InterProIPR014724; RNA_pol_RPB2_OB-fold; .
Gene3DG3DSA:2.30.150.10; G3DSA:2.30.150.10; 1; .
Gene3DG3DSA:2.40.270.10; G3DSA:2.40.270.10; 2; .
Gene3DG3DSA:2.40.50.150; Ribosomal_L2; 2; .
PANTHERPTHR20856:SF3; PTHR20856:SF3; 1; .
PANTHERPTHR20856; RNA_pol_I_sub2; 1; .
PfamPF04563; RNA_pol_Rpb2_1; 1; .
PfamPF04561; RNA_pol_Rpb2_2; 2; .
PfamPF04565; RNA_pol_Rpb2_3; 1; .
PfamPF10385; RNA_pol_Rpb2_45; 1; .
PfamPF00562; RNA_pol_Rpb2_6; 1; .
PfamPF04560; RNA_pol_Rpb2_7; 1; .
TIGRFAMsTIGR02013; RpoB; 1; .
PROSITEPS01166; RNA_POL_BETA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server