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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: RS1_ECOLI




SWISS-2DPAGE: RS1_ECOLI



RS1_ECOLI:

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ID   RS1_ECOLI; STANDARD; 2DG.
AC   P0AG67; P02349;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=30S ribosomal protein S1;.
GN   Name=rpsA; Synonyms=ssyF; OrderedLocusNames=b0911, JW0894;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT   ''''''Protein identifications of O'Farrell two-dimensional gels:
RT   locations of 81 Escherichia coli proteins'';'';'';
RL   J. Bacteriol. 141(1):1409-1420(1980).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=79216190; PubMed=156716; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.;
RT   ''''''Levels of major proteins of Escherichia coli during growth at
RT   different temperatures'';'';'';
RL   J. Bacteriol. 139(1):185-194(1979).
RN   [5]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=78212912; PubMed=352533; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT   ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT   of 140 individual proteins at different growth rates'';'';'';
RL   Cell 14(1):179-190(1978).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [7]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KIV=4.87/67214;
2D   -!-   EXPRESSION: CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS
2D         TEMPERATURE RANGE (23 TO 37C) [4]; INCREASED IN LEVEL WITH INCREASING
2D         GROWTH RATE [5].
2D   -!-   MAPPING: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY
2D         VANBOGELEN [2] AND BLOCH [3].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HFK=4.99/64780;
2D   -!-   MAPPING: GEL MATCHING WITH MASTER ECOLI4.5-5.5 [6].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014SJ=4.99/64780;
2D   -!-   PI/MW: SPOT 2D-00150A=4.98/42474; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014SJ: 1162.3; 1525.45; 1549.34; 1584.33;
2D         1636.37; 1661.33; 3388.5; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-00150A: 1549.71; 1570.66; 1712.81; 1758.78;
2D         1802.68; 2044.89; 2128.9; 2144.92; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [6].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WEH=4.97/68742;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WEH: 1162.66; 1204.7; 1525.96; 1549.87;
2D         1566.79; 1675.85; 1712.97; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [7].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P0AG67; RS1_ECOLI.
DR   ECO2DBASE; B065.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0AG67; RS1_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server