resource logo

SWISS-2DPAGE

Attention: World-2DPAGE is no longer maintained.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: RSUA_ECOLI




SWISS-2DPAGE:  RSUA_ECOLI


RSUA_ECOLI


General information about the entry
View entry in simple text format
Entry nameRSUA_ECOLI
Primary accession numberP0AA43
Secondary accession number(s) P33918
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on November 9, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Ribosomal small subunit pseudouridine synthase A; EC=5.4.99.19; AltName: Full=16S pseudouridylate 516 synthase; AltName: Full=16S rRNA pseudouridine(516) synthase; AltName: Full=rRNA pseudouridylate synthase A; AltName: Full=rRNA-uridine isomerase A;.
Gene nameName=rsuA
Synonyms=yejD
OrderedLocusNames=b2183, JW2171
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.63; Mw=32442  [identification data]
pI=5.78; Mw=32101  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1];
SPOT 2D-001WR8: PEPTIDE MASS FINGERPRINTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP0AA43; RSUA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRSUA_ECOLI
Primary accession numberP0AA43
Secondary accession number(s) P33918 Q2MAQ8
Sequence was last modified on September 13, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 54)
Name and origin of the protein
DescriptionRecName: Full=Ribosomal small subunit pseudouridine synthase A; EC=5.4.99.19; AltName: Full=16S pseudouridylate 516 synthase; AltName: Full=16S rRNA pseudouridine(516) synthase; AltName: Full=rRNA pseudouridylate synthase A; AltName: Full=rRNA-uridine isomerase A;
Gene nameName=rsuA
Synonyms=yejD
OrderedLocusNames=b2183, JW2171
Encoded onName=rsuA; Synonyms=yejD; OrderedLocusNames=b2183, JW2171
Keywords3D-structure; Complete proteome; Direct protein sequencing; Isomerase; Reference proteome; RNA-binding; rRNA processing.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU00008; AAA16377.1; -; Genomic_DNA
EMBLU00096; AAC75244.1; -; Genomic_DNA
EMBLAP009048; BAE76648.1; -; Genomic_DNA
PIRF64987; F64987; .
RefSeqNP_416688.1; NC_000913.2; .
PDB1KSK; X-ray; 2.00 A; A=1-231
PDB1KSL; X-ray; 2.10 A; A=1-231
PDB1KSV; X-ray; 2.65 A; A=1-231
PDBsum1KSK; -; .
PDBsum1KSL; -; .
PDBsum1KSV; -; .
ProteinModelPortalP0AA43; -; .
SMRP0AA43; 1-231; .
DIPDIP-35902N; -; .
IntActP0AA43; 39; .
MINTMINT-1221744; -; .
SWISS-2DPAGEP0AA43; -; .
EnsemblBacteriaEBESCT00000000852; EBESCP00000000852; EBESCG00000000710; .
EnsemblBacteriaEBESCT00000016577; EBESCP00000015868; EBESCG00000015636; .
GeneID945378; -; .
GenomeReviewsAP009048_GR; JW2171; .
GenomeReviewsU00096_GR; b2183; .
KEGGecj:JW2171; -; .
KEGGeco:b2183; -; .
EchoBASEEB1978; -; .
EcoGeneEG12044; rsuA; .
eggNOGCOG1187; -; .
GeneTreeEBGT00050000009689; -; .
HOGENOMHBG754478; -; .
OMAGLHRWKI; -; .
ProtClustDBPRK10839; -; .
BioCycEcoCyc:EG12044-MONOMER; -; .
BioCycMetaCyc:EG12044-MONOMER; -; .
GenevestigatorP0AA43; -; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0009982; F:pseudouridine synthase activity; IEA:InterPro; .
GOGO:0003723; F:RNA binding; IEA:UniProtKB-KW; .
GOGO:0001522; P:pseudouridine synthesis; IEA:InterPro; .
GOGO:0006364; P:rRNA processing; IEA:UniProtKB-KW; .
InterProIPR020103; PsdUridine_synth_cat_dom; .
InterProIPR006145; PsdUridine_synth_RsuA/RluD; .
InterProIPR000748; PsdUridine_synth_RsuA_RluB/E/F; .
InterProIPR018496; PsdUridine_synth_RsuA_RluB_CS; .
InterProIPR002942; S4_RNA-bd; .
PfamPF00849; PseudoU_synth_2; 1; .
PfamPF01479; S4; 1; .
SMARTSM00363; S4; 1; .
SUPFAMSSF55120; PsdUridine_synth_cat_dom; 1; .
TIGRFAMsTIGR00093; TIGR00093; 1; .
PROSITEPS01149; PSI_RSU; 1; .
PROSITEPS50889; S4; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server