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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: SYN_ECOLI |
SWISS-2DPAGE: SYN_ECOLI
SYN_ECOLI:
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ID SYN_ECOLI; STANDARD; 2DG.
AC P0A8M0; P17242;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Asparaginyl-tRNA synthetase; EC=6.1.1.22; AltName:
DE Full=Asparagine--tRNA ligase; Short=AsnRS;.
GN Name=asnS; Synonyms=tss; OrderedLocusNames=b0930, JW0913;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=81069671; PubMed=7002901; [NCBI, Expasy, EBI, Israel, Japan]
RA Phillips T.A., Bloch P.L., Neidhardt F.C.;
RT ''''''Protein identifications on O'Farrell two-dimensional gels:
RT locations of 55 additional Escherichia coli proteins'';'';'';
RL J. Bacteriol. 144(1):1024-1033(1980).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMODIMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KCW=5.64/92858;
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D [2] AND PHILLIPS [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-001533=5.01/39019;
2D -!- PEPTIDE MASSES: SPOT 2D-001533: 914.503; 1011.697; 1773.577;
2D 2066.135; 2661.533; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WIT=4.69/50800;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WIT: (R)VSTLDLENLPR(N),178-188.
2D -!- MAPPING: Tandem mass spectrometry [5].
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DR ECO2DBASE; G099.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A8M0; SYN_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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