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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: SYQ_ECOLI |
SWISS-2DPAGE: SYQ_ECOLI
SYQ_ECOLI:
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ID SYQ_ECOLI; STANDARD; 2DG.
AC P00962;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Glutaminyl-tRNA synthetase; EC=6.1.1.18; AltName:
DE Full=Glutamine--tRNA ligase; Short=GlnRS;.
GN Name=glnS; OrderedLocusNames=b0680, JW0666;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=79216190; PubMed=156716; [NCBI, Expasy, EBI, Israel, Japan]
RA Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.;
RT ''''''Levels of major proteins of Escherichia coli during growth at
RT different temperatures'';'';'';
RL J. Bacteriol. 139(1):185-194(1979).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=78212912; PubMed=352533; [NCBI, Expasy, EBI, Israel, Japan]
RA Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT of 140 individual proteins at different growth rates'';'';'';
RL Cell 14(1):179-190(1978).
RN [6]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: MONOMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KJP=5.88/63346;
2D -!- EXPRESSION: CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS
2D TEMPERATURE RANGE (23 TO 37°C) [4]; INCREASED IN LEVEL WITH INCREASING
2D GROWTH RATE [5].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D [2] AND BLOCH [3].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WE0=5.91/74850;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WE0: (-)SEAEARPTNFIR(Q),1-12.
2D -!- MAPPING: Tandem mass spectrometry [6].
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DR ECO2DBASE; G061.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P00962; SYQ_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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