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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: THIO_HUMAN |
SWISS-2DPAGE: THIO_HUMAN
THIO_HUMAN:
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ID THIO_HUMAN; STANDARD; 2DG.
AC P10599;
DT 01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT 31-MAR-2004, 2D annotation version 5.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Thioredoxin; Short=Trx; AltName: Full=ATL-derived factor;
DE Short=ADF; AltName: Full=Surface-associated sulphydryl protein; Short=SASP;.
GN Name=TXN; Synonyms=TRDX, TRX, TRX1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Hominidae; Homo.
OX NCBI_TaxID=9606;
MT CEC_HUMAN, DLD1_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
MT LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, PLATELET_HUMAN, RBC_HUMAN, U937_HUMAN.
IM CEC_HUMAN, DLD1_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
IM LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, PLATELET_HUMAN, RBC_HUMAN, U937_HUMAN.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA R.D., Hughes G.J.;
RT ''''''Human liver protein map: a reference database established by
RT microsequencing and gel comparison'';'';'';
RL Electrophoresis 13(1):992-1001(1992).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA A., Hochstrasser D.F.;
RT ''''''Inside SWISS-2DPAGE database'';'';'';
RL Electrophoresis 16(1):1131-1151(1995).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA Hochstrasser D.F.;
RT ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL Electrophoresis 14(1):1223-1231(1993).
RN [4]
RP MAPPING ON GEL.
RX MEDLINE=96007935; PubMed=7498158; [NCBI, Expasy, EBI, Israel, Japan]
RA Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant
RA L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.;
RT ''''''Human blood platelet protein map established by two-dimensional
RT polyacrylamide gel electrophoresis'';'';'';
RL Electrophoresis 16(1):1152-1159(1995).
RN [5]
RP MAPPING ON GEL.
RX MEDLINE=97295305; PubMed=9150947; [NCBI, Expasy, EBI, Israel, Japan]
RA Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA Mocarelli P.;
RT ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL Electrophoresis 18(1):599-604(1997).
RN [6]
RP MAPPING ON GEL.
RA Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL Submitted (JUL-1997) to SWISS-2DPAGE.
RN [7]
RP MAPPING ON GEL.
RX MEDLINE=96314065; PubMed=8740185; [NCBI, Expasy, EBI, Israel, Japan]
RA Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA Bladier D., Joubert-Caron R.;
RT ''''''Identification of different galectins by immunoblotting after two-
RT dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL Electrophoresis 17(1):600-606(1996).
RN [8]
RP MAPPING ON GEL.
RX MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
RA Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT ''''''Standardized characterization of gene expression in human
RT colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL Electrophoresis 18(1):2842-2848(1997).
RN [9]
RP MAPPING ON GEL.
RA Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA Appel R.D., Hochstrasser D.F.;
RL Submitted (JAN-1999) to SWISS-2DPAGE.
RN [10]
RP MAPPING ON GEL.
RA Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA P., Tissot J.-D.;
RT ''''''New insights into HIV lymphocyte infection'';'';'';
RL (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC -!- SUBUNIT: SINGLE CHAIN
2D -!- MASTER: CEC_HUMAN;
2D -!- PI/MW: SPOT 2D-000V0S=4.84/11121;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [8].
2D -!- MASTER: DLD1_HUMAN;
2D -!- PI/MW: SPOT 2D-001F9A=4.92/10391;
2D -!- MAPPING: MATCHING WITH THE HEPG2 MASTER GEL [9].
2D -!- MASTER: HEPG2_HUMAN;
2D -!- PI/MW: SPOT 2D-0003ZB=4.92/10936;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D -!- MASTER: HL60_HUMAN;
2D -!- PI/MW: SPOT 2D-00105M=4.89/10727;
2D -!- MAPPING: MATCHING WITH THE KIDNEY MASTER GEL [6][7].
2D -!- MASTER: KIDNEY_HUMAN;
2D -!- PI/MW: SPOT 2D-000O1N=4.88/11271;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000O1N: B=11.9, Z=15.3, S=5.3, H=1,
2D G=7.6, T=4.7, A=8, P=2.3, Y=4.4, R=3.1, V=9, M=1.3, I=5.5, L=7.1, F=6.2, K=7.3;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID
2D COMPOSITION ANALYSIS [5].
2D -!- MASTER: LIVER_HUMAN;
2D -!- PI/MW: SPOT 2D-0001PL=4.89/11907;
2D -!- MAPPING: MICROSEQUENCING [1].
2D -!- MASTER: LYMPHOCYTE_HUMAN;
2D -!- PI/MW: SPOT 2D-001YGA=4.74/16099;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001YGA: (K)TAFQEALDAAGDK(L),8-20.
2D -!- MAPPING: ESI tandem mass spectrometry [10].
2D -!- MASTER: LYMPHOMA_HUMAN;
2D -!- PI/MW: SPOT 2D-0008CT=4.88/11056;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D -!- MASTER: PLATELET_HUMAN;
2D -!- PI/MW: SPOT 2D-000GJE=4.90/11900;
2D -!- MAPPING: MATCHING WITH RBC AND LIVER MASTERS [4].
2D -!- MASTER: RBC_HUMAN;
2D -!- PI/MW: SPOT 2D-0006SV=4.89/12000;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D -!- MASTER: U937_HUMAN;
2D -!- PI/MW: SPOT 2D-000D6S=4.93/10599;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC ---------------------------------------------------------------------------
CC This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
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CC ---------------------------------------------------------------------------
DR DOSAC-COBS 2D-PAGE; P10599; P10599.
DR DOSAC-COBS-2DPAGE; P10599; THIO_HUMAN.
DR PHCI-2DPAGE; P10599; THIO_HUMAN.
DR REPRODUCTION-2DPAGE; IPI00216298; IPI00216298.
DR Siena-2DPAGE; P10599; THIO_HUMAN.
DR UniProtKB/Swiss-Prot; P10599; THIO_HUMAN.
//
The following 2-D maps are available for this protein:
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Colorectal epithelia cells
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Colorectal adenocarcinoma cell line (DL-1)
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HepG2
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Promyelocytic leukemia cells
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Kidney
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Liver
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Lymphocytes
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Lymphoma
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Platelet
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Red blood cells
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Macrophage Like Cell Line
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SWISS-2DPAGE (search AC)
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