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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: TOP1_HUMAN



General information about the entry
View entry in simple text format
Entry nameTOP1_HUMAN
Primary accession numberP11387
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 4)
Name and origin of the protein
DescriptionRecName: Full=DNA topoisomerase 1; EC=; AltName: Full=DNA topoisomerase I;.
Gene nameName=TOP1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
  • SUBUNIT: Monomer
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
  map experimental info
  protein estimated location

Mw=96301  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP11387; TOP1_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTOP1_HUMAN
Primary accession numberP11387
Secondary accession number(s) A8KA78 E1P5W3 O43256 Q12855 Q12856 Q15610 Q5TFY3 Q9UJN0
Sequence was last modified on December 1, 2000 (version 2)
Annotations were last modified on October 19, 2011 (version 139)
Name and origin of the protein
DescriptionRecName: Full=DNA topoisomerase 1; EC=; AltName: Full=DNA topoisomerase I;
Gene nameName=TOP1
Encoded onName=TOP1
Keywords3D-structure; Acetylation; ATP-binding; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; DNA-binding; Host-virus interaction; Isomerase; Isopeptide bond; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Topoisomerase; Ubl conjugation.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLJ03250; AAA61207.1; -; mRNA
EMBLM60706; AAA61206.1; -; Genomic_DNA
EMBLM60688; AAA61206.1; JOINED; Genomic_DNA
EMBLM60689; AAA61206.1; JOINED; Genomic_DNA
EMBLM60690; AAA61206.1; JOINED; Genomic_DNA
EMBLM60691; AAA61206.1; JOINED; Genomic_DNA
EMBLM60692; AAA61206.1; JOINED; Genomic_DNA
EMBLM60693; AAA61206.1; JOINED; Genomic_DNA
EMBLM60694; AAA61206.1; JOINED; Genomic_DNA
EMBLM60695; AAA61206.1; JOINED; Genomic_DNA
EMBLM60696; AAA61206.1; JOINED; Genomic_DNA
EMBLM60697; AAA61206.1; JOINED; Genomic_DNA
EMBLM60698; AAA61206.1; JOINED; Genomic_DNA
EMBLM60699; AAA61206.1; JOINED; Genomic_DNA
EMBLM60700; AAA61206.1; JOINED; Genomic_DNA
EMBLM60701; AAA61206.1; JOINED; Genomic_DNA
EMBLM60702; AAA61206.1; JOINED; Genomic_DNA
EMBLM60703; AAA61206.1; JOINED; Genomic_DNA
EMBLM60704; AAA61206.1; JOINED; Genomic_DNA
EMBLM60705; AAA61206.1; JOINED; Genomic_DNA
EMBLAK292943; BAF85632.1; -; mRNA
EMBLAL035652; CAI19767.1; -; Genomic_DNA
EMBLAL022394; CAI19767.1; JOINED; Genomic_DNA
EMBLAL022394; CAI42886.1; -; Genomic_DNA
EMBLAL035652; CAI42886.1; JOINED; Genomic_DNA
EMBLCH471077; EAW75994.1; -; Genomic_DNA
EMBLCH471077; EAW75995.1; -; Genomic_DNA
EMBLX52601; CAA36834.1; ALT_SEQ; Genomic_DNA
EMBLU07804; AAB60379.1; -; mRNA
EMBLU07806; AAB60380.1; -; mRNA
EMBLX16479; CAA34500.2; -; mRNA
EMBLM27913; AAA61208.1; -; mRNA
IPIIPI00413611; -; .
PIRA30887; ISHUT1; .
RefSeqNP_003277.1; NM_003286.2; .
UniGeneHs.472737; -; .
PDB1A31; X-ray; 2.10 A; A=175-765
PDB1A35; X-ray; 2.50 A; A=175-765
PDB1A36; X-ray; 2.80 A; A=175-765
PDB1EJ9; X-ray; 2.60 A; A=203-765
PDB1K4S; X-ray; 3.20 A; A=174-765
PDB1K4T; X-ray; 2.10 A; A=174-765
PDB1LPQ; X-ray; 3.14 A; A=202-765
PDB1NH3; X-ray; 3.10 A; A=203-764
PDB1R49; X-ray; 3.13 A; A=174-765
PDB1RR8; X-ray; 2.60 A; C=203-765
PDB1RRJ; X-ray; 2.30 A; A=201-765
PDB1SC7; X-ray; 3.00 A; A=174-765
PDB1SEU; X-ray; 3.00 A; A=174-765
PDB1T8I; X-ray; 3.00 A; A=174-765
PDB1TL8; X-ray; 3.10 A; A=174-765
PDBsum1A31; -; .
PDBsum1A35; -; .
PDBsum1A36; -; .
PDBsum1EJ9; -; .
PDBsum1K4S; -; .
PDBsum1K4T; -; .
PDBsum1LPQ; -; .
PDBsum1NH3; -; .
PDBsum1R49; -; .
PDBsum1RR8; -; .
PDBsum1RRJ; -; .
PDBsum1SC7; -; .
PDBsum1SEU; -; .
PDBsum1T8I; -; .
PDBsum1TL8; -; .
ProteinModelPortalP11387; -; .
SMRP11387; 201-765; .
DisProtDP00075; -; .
IntActP11387; 44; .
MINTMINT-94018; -; .
STRINGP11387; -; .
PhosphoSiteP11387; -; .
SWISS-2DPAGEP11387; -; .
Aarhus/Ghent-2DPAGE610; NEPHGE; .
PeptideAtlasP11387; -; .
PRIDEP11387; -; .
EnsemblENST00000361337; ENSP00000354522; ENSG00000198900; .
GeneID7150; -; .
KEGGhsa:7150; -; .
UCSCuc002xjl.1; human; .
CTD7150; -; .
GeneCardsGC20P036395; -; .
H-InvDBHIX0015818; -; .
HGNCHGNC:11986; TOP1; .
HPACAB009058; -; .
HPAHPA019039; -; .
MIM126420; gene; .
neXtProtNX_P11387; -; .
PharmGKBPA353; -; .
eggNOGprNOG05347; -; .
HOVERGENHBG007988; -; .
InParanoidP11387; -; .
OrthoDBEOG4V170F; -; .
PhylomeDBP11387; -; .
Pathway_Interaction_DBcaspase_pathway; Caspase cascade in apoptosis; .
DrugBankDB00762; Irinotecan; .
DrugBankDB04967; Lucanthone; .
DrugBankDB01030; Topotecan; .
NextBio27982; -; .
PMAP-CutDBP11387; -; .
ArrayExpressP11387; -; .
BgeeP11387; -; .
CleanExHS_TOP1; -; .
GenevestigatorP11387; -; .
GermOnlineENSG00000198900; Homo sapiens; .
GOGO:0005694; C:chromosome; IEA:InterPro; .
GOGO:0005730; C:nucleolus; IDA:UniProtKB; .
GOGO:0005654; C:nucleoplasm; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0031490; F:chromatin DNA binding; IBA:RefGenome; .
GOGO:0003918; F:DNA topoisomerase (ATP-hydrolyzing) activity; IEA:InterPro; .
GOGO:0003917; F:DNA topoisomerase type I activity; IMP:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0006265; P:DNA topological change; IBA:RefGenome; .
GOGO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW; .
GOGO:0016310; P:phosphorylation; NAS:UniProtKB; .
GOGO:0012501; P:programmed cell death; NAS:UniProtKB; .
GOGO:0042493; P:response to drug; IEP:UniProtKB; .
InterProIPR011010; DNA_brk_join_enz; .
InterProIPR013034; DNA_topo_domain1; .
InterProIPR018521; TopoI_AS; .
InterProIPR001631; TopoI_C; .
InterProIPR013499; TopoI_C_euk; .
InterProIPR014711; TopoI_cat_a-hlx-sub_euk; .
InterProIPR014727; TopoI_cat_a/b-sub_euk; .
InterProIPR013500; TopoI_cat_euk; .
InterProIPR008336; TopoI_DNA-bd_euk; .
InterProIPR013030; TopoI_DNA-bd_mixed-a/b_euk; .
InterProIPR009054; TopoI_insert_euk; .
Gene3DG3DSA:; TopoI_cat_a-hlx-sub_euk; 1; .
Gene3DG3DSA:; TopoI_cat_a/b-sub_euk; 1; .
Gene3DG3DSA:; TopoI_DNA-bd_a-hlx_euk; 1; .
Gene3DG3DSA:; TopoI_DNA-bd_mixed-a/b_euk; 2; .
PfamPF01028; Topoisom_I; 1; .
PfamPF02919; Topoisom_I_N; 1; .
SMARTSM00435; TOPEUc; 1; .
SUPFAMSSF56349; DNA_brk_join_enz; 1; .
SUPFAMSSF46596; Topismrse_insert; 1; .
SUPFAMSSF56741; TopoI_DNA_bd_euk; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server