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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: TOP1_HUMAN SWISS-2DPAGE:  TOP1_HUMAN TOP1_HUMAN General information about the entry View entry in simple text format Entry name TOP1_HUMAN Primary accession number P11387 integrated into SWISS-2DPAGE on  May 15, 2003 (release 16) 2D Annotations were last modified on May 15, 2003 (version 1) General Annotations were last modified on  May 19, 2011 (version 4) Name and origin of the protein Description RecName: Full=DNA topoisomerase 1; EC=5.99.1.2; AltName: Full=DNA topoisomerase I;. Gene name Name=TOP1 Annotated species Homo sapiens (Human) [TaxID: 9606] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. References [1]   MAPPING ON GEL PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan] Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. ''''''Functional proteomic analysis of human nucleolus'';'';'' Mol. Biol. Cell. 13(1):4100-4109(2002) Comments SUBUNIT: Monomer 2D PAGE maps for identified proteins How to interpret a protein NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells} Homo sapiens (Human) Tissue: Cervix carcinoma   map experimental info   protein estimated location   NUCLEOLI_HELA_1D_HUMAN MAP LOCATIONS: BAND 1D-001V7Y: Mw=96301   [identification data] MAPPING (identification): Tandem mass spectrometry [1]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references UniProtKB/Swiss-Prot P11387; TOP1_HUMAN. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name TOP1_HUMAN Primary accession number P11387 Secondary accession number(s) A8KA78 E1P5W3 O43256 Q12855 Q12856 Q15610 Q5TFY3 Q9UJN0 Sequence was last modified on  December 1, 2000 (version 2) Annotations were last modified on  October 19, 2011 (version 139) Name and origin of the protein Description RecName: Full=DNA topoisomerase 1; EC=5.99.1.2; AltName: Full=DNA topoisomerase I; Gene name Name=TOP1 Encoded on Name=TOP1 Keywords 3D-structure; Acetylation; ATP-binding; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; DNA-binding; Host-virus interaction; Isomerase; Isopeptide bond; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Topoisomerase; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL J03250; AAA61207.1; -; mRNA EMBL M60706; AAA61206.1; -; Genomic_DNA EMBL M60688; AAA61206.1; JOINED; Genomic_DNA EMBL M60689; AAA61206.1; JOINED; Genomic_DNA EMBL M60690; AAA61206.1; JOINED; Genomic_DNA EMBL M60691; AAA61206.1; JOINED; Genomic_DNA EMBL M60692; AAA61206.1; JOINED; Genomic_DNA EMBL M60693; AAA61206.1; JOINED; Genomic_DNA EMBL M60694; AAA61206.1; JOINED; Genomic_DNA EMBL M60695; AAA61206.1; JOINED; Genomic_DNA EMBL M60696; AAA61206.1; JOINED; Genomic_DNA EMBL M60697; AAA61206.1; JOINED; Genomic_DNA EMBL M60698; AAA61206.1; JOINED; Genomic_DNA EMBL M60699; AAA61206.1; JOINED; Genomic_DNA EMBL M60700; AAA61206.1; JOINED; Genomic_DNA EMBL M60701; AAA61206.1; JOINED; Genomic_DNA EMBL M60702; AAA61206.1; JOINED; Genomic_DNA EMBL M60703; AAA61206.1; JOINED; Genomic_DNA EMBL M60704; AAA61206.1; JOINED; Genomic_DNA EMBL M60705; AAA61206.1; JOINED; Genomic_DNA EMBL AK292943; BAF85632.1; -; mRNA EMBL AL035652; CAI19767.1; -; Genomic_DNA EMBL AL022394; CAI19767.1; JOINED; Genomic_DNA EMBL AL022394; CAI42886.1; -; Genomic_DNA EMBL AL035652; CAI42886.1; JOINED; Genomic_DNA EMBL CH471077; EAW75994.1; -; Genomic_DNA EMBL CH471077; EAW75995.1; -; Genomic_DNA EMBL X52601; CAA36834.1; ALT_SEQ; Genomic_DNA EMBL U07804; AAB60379.1; -; mRNA EMBL U07806; AAB60380.1; -; mRNA EMBL X16479; CAA34500.2; -; mRNA EMBL M27913; AAA61208.1; -; mRNA IPI IPI00413611; -; . PIR A30887; ISHUT1; . RefSeq NP_003277.1; NM_003286.2; . UniGene Hs.472737; -; . PDB 1A31; X-ray; 2.10 A; A=175-765 PDB 1A35; X-ray; 2.50 A; A=175-765 PDB 1A36; X-ray; 2.80 A; A=175-765 PDB 1EJ9; X-ray; 2.60 A; A=203-765 PDB 1K4S; X-ray; 3.20 A; A=174-765 PDB 1K4T; X-ray; 2.10 A; A=174-765 PDB 1LPQ; X-ray; 3.14 A; A=202-765 PDB 1NH3; X-ray; 3.10 A; A=203-764 PDB 1R49; X-ray; 3.13 A; A=174-765 PDB 1RR8; X-ray; 2.60 A; C=203-765 PDB 1RRJ; X-ray; 2.30 A; A=201-765 PDB 1SC7; X-ray; 3.00 A; A=174-765 PDB 1SEU; X-ray; 3.00 A; A=174-765 PDB 1T8I; X-ray; 3.00 A; A=174-765 PDB 1TL8; X-ray; 3.10 A; A=174-765 PDBsum 1A31; -; . PDBsum 1A35; -; . PDBsum 1A36; -; . PDBsum 1EJ9; -; . PDBsum 1K4S; -; . PDBsum 1K4T; -; . PDBsum 1LPQ; -; . PDBsum 1NH3; -; . PDBsum 1R49; -; . PDBsum 1RR8; -; . PDBsum 1RRJ; -; . PDBsum 1SC7; -; . PDBsum 1SEU; -; . PDBsum 1T8I; -; . PDBsum 1TL8; -; . ProteinModelPortal P11387; -; . SMR P11387; 201-765; . DisProt DP00075; -; . IntAct P11387; 44; . MINT MINT-94018; -; . STRING P11387; -; . PhosphoSite P11387; -; . SWISS-2DPAGE P11387; -; . Aarhus/Ghent-2DPAGE 610; NEPHGE; . PeptideAtlas P11387; -; . PRIDE P11387; -; . Ensembl ENST00000361337; ENSP00000354522; ENSG00000198900; . GeneID 7150; -; . KEGG hsa:7150; -; . UCSC uc002xjl.1; human; . CTD 7150; -; . GeneCards GC20P036395; -; . H-InvDB HIX0015818; -; . HGNC HGNC:11986; TOP1; . HPA CAB009058; -; . HPA HPA019039; -; . MIM 126420; gene; . neXtProt NX_P11387; -; . PharmGKB PA353; -; . eggNOG prNOG05347; -; . HOVERGEN HBG007988; -; . InParanoid P11387; -; . OMA ESVKFYY; -; . OrthoDB EOG4V170F; -; . PhylomeDB P11387; -; . Pathway_Interaction_DB caspase_pathway; Caspase cascade in apoptosis; . DrugBank DB00762; Irinotecan; . DrugBank DB04967; Lucanthone; . DrugBank DB01030; Topotecan; . NextBio 27982; -; . PMAP-CutDB P11387; -; . ArrayExpress P11387; -; . Bgee P11387; -; . CleanEx HS_TOP1; -; . Genevestigator P11387; -; . GermOnline ENSG00000198900; Homo sapiens; . GO GO:0005694; C:chromosome; IEA:InterPro; . GO GO:0005730; C:nucleolus; IDA:UniProtKB; . GO GO:0005654; C:nucleoplasm; IDA:UniProtKB; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0031490; F:chromatin DNA binding; IBA:RefGenome; . GO GO:0003918; F:DNA topoisomerase (ATP-hydrolyzing) activity; IEA:InterPro; . GO GO:0003917; F:DNA topoisomerase type I activity; IMP:UniProtKB; . GO GO:0005515; F:protein binding; IPI:UniProtKB; . GO GO:0006265; P:DNA topological change; IBA:RefGenome; . GO GO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW; . GO GO:0016310; P:phosphorylation; NAS:UniProtKB; . GO GO:0012501; P:programmed cell death; NAS:UniProtKB; . GO GO:0042493; P:response to drug; IEP:UniProtKB; . InterPro IPR011010; DNA_brk_join_enz; . InterPro IPR013034; DNA_topo_domain1; . InterPro IPR018521; TopoI_AS; . InterPro IPR001631; TopoI_C; . InterPro IPR013499; TopoI_C_euk; . InterPro IPR014711; TopoI_cat_a-hlx-sub_euk; . InterPro IPR014727; TopoI_cat_a/b-sub_euk; . InterPro IPR013500; TopoI_cat_euk; . InterPro IPR008336; TopoI_DNA-bd_euk; . InterPro IPR013030; TopoI_DNA-bd_mixed-a/b_euk; . InterPro IPR009054; TopoI_insert_euk; . Gene3D G3DSA:3.90.15.10; TopoI_cat_a-hlx-sub_euk; 1; . Gene3D G3DSA:1.10.132.10; TopoI_cat_a/b-sub_euk; 1; . Gene3D G3DSA:1.10.10.41; TopoI_DNA-bd_a-hlx_euk; 1; . Gene3D G3DSA:2.170.11.10; TopoI_DNA-bd_mixed-a/b_euk; 2; . Pfam PF01028; Topoisom_I; 1; . Pfam PF02919; Topoisom_I_N; 1; . PRINTS PR00416; EUTPISMRASEI; . SMART SM00435; TOPEUc; 1; . SUPFAM SSF56349; DNA_brk_join_enz; 1; . SUPFAM SSF46596; Topismrse_insert; 1; . SUPFAM SSF56741; TopoI_DNA_bd_euk; 1; . PROSITE PS00176; TOPOISOMERASE_I_EUK; 1; . SWISS-2DPAGE (search AC)