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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: TPIS_YEAST




SWISS-2DPAGE:  TPIS_YEAST


TPIS_YEAST


General information about the entry
View entry in simple text format
Entry nameTPIS_YEAST
Primary accession numberP00942
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase;.
Gene nameName=TPI1
OrderedLocusNames=YDR050C
ORFNames=YD9609.05C
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=82271855; PubMed=7050667; [NCBI, Expasy, EBI, Israel, Japan]
Ludwig J.R., Foy J.J., Elliott S.G., McLaughlin C.S.
''''''Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae'';'';''
Mol. Cell. Biol. 2(1):117-126(1982)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=6.01; Mw=23175
pI=5.64; Mw=23058

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY LUDWIG ET AL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP00942; TPIS_YEAST.
UniProtKB/Swiss-ProtP00942; TPIS_YEAST.
YEPD6220.
YEPD6230.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTPIS_YEAST
Primary accession numberP00942
Secondary accession number(s) D6VS37
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 133)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase;
Gene nameName=TPI1
OrderedLocusNames=YDR050C
ORFNames=YD9609.05C
Encoded onName=TPI1; OrderedLocusNames=YDR050C; ORFNames=YD9609.05C
Keywords3D-structure; Complete proteome; Direct protein sequencing; Gluconeogenesis; Glycolysis; Isomerase; Pentose shunt; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01366; AAA88757.1; -; Genomic_DNA
EMBLZ49209; CAA89080.1; -; Genomic_DNA
EMBLAY557654; AAS55980.1; -; Genomic_DNA
EMBLBK006938; DAA11897.1; -; Genomic_DNA
PIRA01168; ISBYT; .
RefSeqNP_010335.1; NM_001180358.1; .
PDB1I45; X-ray; 1.80 A; A/B=2-247
PDB1NEY; X-ray; 1.20 A; A/B=2-248
PDB1NF0; X-ray; 1.60 A; A/B=2-248
PDB1YPI; X-ray; 1.90 A; A/B=2-248
PDB2YPI; X-ray; 2.50 A; A/B=2-248
PDB3YPI; X-ray; 2.80 A; A/B=2-247
PDB7TIM; X-ray; 1.90 A; A/B=2-247
PDBsum1I45; -; .
PDBsum1NEY; -; .
PDBsum1NF0; -; .
PDBsum1YPI; -; .
PDBsum2YPI; -; .
PDBsum3YPI; -; .
PDBsum7TIM; -; .
ProteinModelPortalP00942; -; .
SMRP00942; 2-248; .
DisProtDP00430; -; .
DIPDIP-6671N; -; .
IntActP00942; 6; .
MINTMINT-614733; -; .
STRINGP00942; -; .
SWISS-2DPAGEP00942; -; .
COMPLUYEAST-2DPAGEP00942; -; .
PeptideAtlasP00942; -; .
EnsemblFungiYDR050C; YDR050C; YDR050C; .
GeneID851620; -; .
KEGGsce:YDR050C; -; .
NMPDRfig|4932.3.peg.1080; -; .
SGDS000002457; TPI1; .
eggNOGfuNOG04390; -; .
GeneTreeEFGT00050000003545; -; .
HOGENOMHBG708281; -; .
OMARAYHRES; -; .
OrthoDBEOG479JH2; -; .
PhylomeDBP00942; -; .
NextBio969151; -; .
ArrayExpressP00942; -; .
GenevestigatorP00942; -; .
GermOnlineYDR050C; Saccharomyces cerevisiae; .
GOGO:0005739; C:mitochondrion; IDA:SGD; .
GOGO:0001950; C:plasma membrane enriched fraction; IDA:SGD; .
GOGO:0004807; F:triose-phosphate isomerase activity; IDA:SGD; .
GOGO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW; .
GOGO:0006096; P:glycolysis; IMP:SGD; .
GOGO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-KW; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR022896; TrioseP_Isoase_bac/euk; .
InterProIPR000652; Triosephosphate_isomerase; .
InterProIPR020861; Triosephosphate_isomerase_AS; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PANTHERPTHR21139; Triophos_ismrse; 1; .
PfamPF00121; TIM; 1; .
SUPFAMSSF51351; Triophos_ismrse; 1; .
TIGRFAMsTIGR00419; Tim; 1; .
PROSITEPS00171; TIM_1; 1; .
PROSITEPS51440; TIM_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server