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SWISS-2DPAGE

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It will be discontinued on 31-May-2024.

Swiss-2DPAGE data (text records and image files) will continue to be available from https://ftp.expasy.org/databases/swiss-2dpage/.


SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: TRPB_ECOLI




SWISS-2DPAGE:  TRPB_ECOLI


TRPB_ECOLI


General information about the entry
View entry in simple text format
Entry nameTRPB_ECOLI
Primary accession numberP0A879
Secondary accession number(s) P00932
integrated into SWISS-2DPAGE on April 1, 2000 (release 12)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Tryptophan synthase beta chain; EC=4.2.1.20;.
Gene nameName=trpB
OrderedLocusNames=b1261, JW1253
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.03; Mw=33559  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.75; Mw=41241  [identification data]

MAPPING (identification):
Tandem mass spectrometry [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A879; TRPB_ECOLI.
ECO2DBASEG040.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0A879; TRPB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTRPB_ECOLI
Primary accession numberP0A879
Secondary accession number(s) P00932 P78146
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 67)
Name and origin of the protein
DescriptionRecName: Full=Tryptophan synthase beta chain; EC=4.2.1.20;
Gene nameName=trpB
OrderedLocusNames=b1261, JW1253
Encoded onName=trpB; OrderedLocusNames=b1261, JW1253
Keywords3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Complete proteome; Direct protein sequencing; Lyase; Pyridoxal phosphate; Reference proteome; Tryptophan biosynthesis.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00365; CAA23663.1; -; Genomic_DNA
EMBLV00372; CAA23674.1; -; Genomic_DNA
EMBLU00096; AAC74343.1; -; Genomic_DNA
EMBLAP009048; BAA14793.1; -; Genomic_DNA
EMBLJ01714; AAA57300.1; -; Genomic_DNA
EMBLU23489; AAB60034.1; -; Genomic_DNA
EMBLU23490; AAA65139.1; -; Genomic_DNA
EMBLU23492; AAA65151.1; -; Genomic_DNA
EMBLU23493; AAA65157.1; -; Genomic_DNA
EMBLU23500; AAA65175.1; -; Genomic_DNA
EMBLU25417; AAA73790.1; -; Genomic_DNA
EMBLU25418; AAA73796.1; -; Genomic_DNA
EMBLU25419; AAA73802.1; -; Genomic_DNA
EMBLU25422; AAA73820.1; -; Genomic_DNA
EMBLU25423; AAA73826.1; -; Genomic_DNA
EMBLU25426; AAA73838.1; -; Genomic_DNA
EMBLU25427; AAA73844.1; -; Genomic_DNA
EMBLU25428; AAA73850.1; -; Genomic_DNA
EMBLU25429; AAA73856.1; -; Genomic_DNA
PIRH64873; TSECB; .
RefSeqNP_415777.1; NC_000913.2; .
PDB2DH5; X-ray; 2.90 A; A=1-397
PDB2DH6; X-ray; 3.00 A; A=1-397
PDBsum2DH5; -; .
PDBsum2DH6; -; .
ProteinModelPortalP0A879; -; .
SMRP0A879; 3-397; .
IntActP0A879; 6; .
SWISS-2DPAGEP0A879; -; .
2DBase-EcoliP0A879; -; .
ECO2DBASEG040.0; 6TH EDITION; .
EnsemblBacteriaEBESCT00000004137; EBESCP00000004137; EBESCG00000003377; .
EnsemblBacteriaEBESCT00000004138; EBESCP00000004138; EBESCG00000003377; .
EnsemblBacteriaEBESCT00000015265; EBESCP00000014556; EBESCG00000014325; .
GeneID945768; -; .
GenomeReviewsAP009048_GR; JW1253; .
GenomeReviewsU00096_GR; b1261; .
KEGGecj:JW1253; -; .
KEGGeco:b1261; -; .
EchoBASEEB1018; -; .
EcoGeneEG11025; trpB; .
eggNOGCOG0133; -; .
GeneTreeEBGT00050000009526; -; .
HOGENOMHBG303148; -; .
OMALKREDLC; -; .
ProtClustDBPRK04346; -; .
BioCycEcoCyc:TRYPSYN-BPROTEIN; -; .
BioCycMetaCyc:TRYPSYN-BPROTEIN; -; .
GenevestigatorP0A879; -; .
GOGO:0005737; C:cytoplasm; IDA:EcoliWiki; .
GOGO:0042803; F:protein homodimerization activity; IDA:EcoCyc; .
GOGO:0030170; F:pyridoxal phosphate binding; IDA:EcoliWiki; .
GOGO:0004834; F:tryptophan synthase activity; IDA:EcoCyc; .
HAMAPMF_00133; Trp_synth_beta; 1; -
InterProIPR001926; PyrdxlP-dep_enz_bsu; .
InterProIPR006653; Trp_synth_b_CS; .
InterProIPR006654; Trp_synth_beta; .
InterProIPR023026; Trp_synth_beta/beta-like; .
PANTHERPTHR10314:SF3; PTHR10314:SF3; 1; .
PfamPF00291; PALP; 1; .
PIRSFPIRSF001413; Trp_syn_beta; 1; .
SUPFAMSSF53686; PyrdxlP-dep_enz_bsu; 1; .
TIGRFAMsTIGR00263; TrpB; 1; .
PROSITEPS00168; TRP_SYNTHASE_BETA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server