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SWISS-2DPAGE

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It will be discontinued on 31-May-2024.

Swiss-2DPAGE data (text records and image files) will continue to be available from https://ftp.expasy.org/databases/swiss-2dpage/.


SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: UBC_HUMAN




SWISS-2DPAGE: UBC_HUMAN



UBC_HUMAN:

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ID   UBC_HUMAN; STANDARD; 2DG.
AC   P0CG48; P02248; P62988;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   15-MAY-2003, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 16.
DE   RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor;.
GN   Name=UBC;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
MT   LIVER_HUMAN, NUCLEOLI_HELA_1D_HUMAN, PLATELET_HUMAN, RBC_HUMAN, U937_HUMAN.
IM   DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
IM   LIVER_HUMAN, NUCLEOLI_HELA_1D_HUMAN, PLATELET_HUMAN, RBC_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=96007935; PubMed=7498158; [NCBI, Expasy, EBI, Israel, Japan]
RA   Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant
RA   L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.;
RT   ''''''Human blood platelet protein map established by two-dimensional
RT   polyacrylamide gel electrophoresis'';'';'';
RL   Electrophoresis 16(1):1152-1159(1995).
RN   [5]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [6]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [7]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, Expasy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [8]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [9]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V9W=24176;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V9W: (K)TITLEVEPSDTIENVK(A),12-27.
1D   -!-   MAPPING: Tandem mass spectrometry [9].
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001FBL=6.85/8761;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001FBL: 1039.35; 1067.45; 1081.39; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [8].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000EUT=6.95/6528;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00040Z=6.89/8800;
2D   -!-   MAPPING: MICROSEQUENCING AND MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00109F=6.90/8565;
2D   -!-   MAPPING: MATCHING WITH THE KIDNEY MASTER GEL [6][7].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000O34=7.18/9474;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [5].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001T1=6.90/8818;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: PLATELET_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000GJA=7.83/12079;
2D   -!-   MAPPING: MATCHING WITH RBC AND LIVER MASTERS [4].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0006UB=6.93/10383;
2D   -!-   PI/MW: SPOT 2D-0006UE=6.99/10149;
2D   -!-   PI/MW: SPOT 2D-0006VT=6.96/8137;
2D   -!-   PI/MW: SPOT 2D-0006W5=7.11/7656;
2D   -!-   PI/MW: SPOT 2D-0006XA=7.99/6377;
2D   -!-   MAPPING: MICROSEQUENCING [3].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D76=6.85/8881;
2D   -!-   PI/MW: SPOT 2D-000D77=6.90/8844;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS-2DPAGE; P62988; UBIQ_HUMAN.
DR   HSC-2DPAGE; P02248; HUMAN.
DR   Siena-2DPAGE; P02248; UBIQ_HUMAN.
DR   UCD-2DPAGE; P02248; UBC_HUMAN.
DR   UniProtKB/Swiss-Prot; P0CG48; UBC_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2
HEPG2_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Liver
LIVER_HUMAN
SDS-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_1D_HUMAN
Platelet
PLATELET_HUMAN
Red blood cells
RBC_HUMAN
Macrophage Like Cell Line
U937_HUMAN
  


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server