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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ZNUA_ECOLI




SWISS-2DPAGE: ZNUA_ECOLI



ZNUA_ECOLI:

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ID   ZNUA_ECOLI; STANDARD; 2DG.
AC   P39172;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=High-affinity zinc uptake system protein znuA; Flags: Precursor;.
GN   Name=znuA; Synonyms=yebL, yzzP; OrderedLocusNames=b1857, JW5831;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L14=4.80/40251;
2D   -!-   PI/MW: SPOT 2D-000L8G=5.41/32899;
2D   -!-   PI/MW: SPOT 2D-000L93=5.39/31992;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000L8G: B=10.3, Z=14.1, S=6.4,
2D         H=1.9, G=4.8, T=7.6, A=9.8, P=5.6, Y=2, R=5.6, V=6.9, M=0.9, I=3, L=9.8,
2D         F=5, K=6.3;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000L93: B=8.6, Z=11.5, S=6.6,
2D         H=1.8, G=9.2, T=5.6, A=10.5, P=6.7, Y=1.8, R=4.3, V=8.2, M=2.6, I=4.3,
2D         L=9, F=4.8, K=4.2;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (AVVA) [1].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-00155Y=5.58/34287;
2D   -!-   PEPTIDE MASSES: SPOT 2D-00155Y: 1088.559; 1842.094; 2261.225;
2D         2407.332; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LC7=5.51/37702;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LC7: 1072.688; 1088.675; 1259.768;
2D         1590.779; 1742.927; 1826.185; 1842.178; 2234.329; 2261.296; 2407.48;
2D         2643.451; 2659.428; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WQO=5.47/32751;
2D   -!-   PI/MW: SPOT 2D-001WQW=5.49/32476;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WQO: (K)QVTIAQLEDVKPLLMK(S),101-116;
2D         (R)ATAVAIHGK(L),153-161; (K)GYFVFHDAYGYFEK(Q),202-215;
2D         (K)QFGLTPLGHFTVNPEIQPGAQR(L),216-237; (K)ATCVFAEPQFRPAVVESVAR(G),250-269.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WQW: (K)ATCVFAEPQFRPAVVESVAR(G),250-269.
2D   -!-   EXPRESSION: decrease after benzoic acid treatment [3].
2D   -!-   MAPPING: Tandem mass spectrometry [3].
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DR   2DBase-Ecoli; P39172; ZNUA_ECOLI.
DR   UniProtKB/Swiss-Prot; P39172; ZNUA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server